Department of Microbiology, University of Stellenbosch, Matieland, Stellenbosch, 7602, South Africa.
Department of Biochemistry, Stellenbosch University, Stellenbosch, South Africa.
Curr Microbiol. 2020 Mar;77(3):369-387. doi: 10.1007/s00284-019-01797-3. Epub 2019 Dec 12.
Bacteria isolated from different segments of the gastro-intestinal tract (GIT) of healthy free-range broilers were screened for probiotic properties. Six strains were selected and identified as Lactobacillus gallinarum, Lactobacillus johnsonii, Lactobacillus salivarius, Lactobacillus crispatus, Enterococcus faecalis and Bacillus amyloliquefaciens based on 16S rRNA, gyrB and recA gene sequence analyses. All six strains produced exopolysaccharides (EPS) and formed biofilms under conditions simulating the broiler GIT. Lactobacillus johnsonii DPN184 and L. salivarius DPN181 produced hydrogen peroxide, and L. crispatus DPN167 and E. faecalis DPN94 produced bile salt hydrolase (BSH) and phytase. Bacillus amyloliquefaciens DPN123 produced phytase, amylase, surfactin and iturin A1. No abnormalities were observed when broilers were fed the multi-strain combination, suggesting that it could be used as a probiotic.
从健康自由放养肉鸡的胃肠道（GIT）不同部位分离出的细菌被筛选出具有益生菌特性。根据 16S rRNA、gyrB 和 recA 基因序列分析，选择并鉴定了 6 株菌，分别为鸡源乳杆菌、约氏乳杆菌、唾液乳杆菌、卷曲乳杆菌、粪肠球菌和解淀粉芽孢杆菌。所有 6 株菌均能在模拟肉鸡 GIT 条件下产生胞外多糖（EPS）并形成生物膜。约氏乳杆菌 DPN184 和唾液乳杆菌 DPN181 产生过氧化氢，卷曲乳杆菌 DPN167 和粪肠球菌 DPN94 产生胆盐水解酶（BSH）和植酸酶。解淀粉芽孢杆菌 DPN123 产生植酸酶、淀粉酶、表面活性剂和伊枯草菌素 A1。当肉鸡喂食多菌株组合时未观察到异常，表明其可用作益生菌。
