Danacioglu Yavuz Onur, Erol Bulent, Ozkanli Seyma, Yildirim Asif, Atis Ramazan Gokhan, Silay Mesrur Selcuk, Caskurlu Turhan
Department of Urology, Istanbul Medeniyet University Faculty of Medicine, Goztepe Training & Research Hospital, Istanbul, Turkey.
Department of Pathology, Istanbul Medeniyet University Faculty of Medicine, Goztepe Training & Research Hospital, Istanbul, Turkey.
Int Neurourol J. 2021 Mar;25(1):42-50. doi: 10.5213/inj.1938176.088. Epub 2021 Jan 19.
Three intravesical treatment agents were compared in an interstitial cystitis rat model: chondroitin sulfate, hyaluronic acid, and combined hyaluronic acid-chondroitin sulfate.
Thirty-five female rats were divided into 5 groups: control (group I), isotonic (group II), chondroitin sulfate (group III), hyaluronic acid (group IV), and hyaluronic acid-chondroitin sulfate (group V). Chemical cystitis was induced in all experimental groups by intravesical instillation of 1 mL of hydrogen peroxide (H2O2) for 15 minutes via the transurethral route. The treatment was administered every other day for 3 sessions 2 days after inducing chemical cystitis. Groups II, III, IV, and V received 1 mL of 0.9% NaCl, 1 mL of 0.2% sodium chondroitin sulfate, 1 mL of low-molecular-weight hyaluronic acid, and 1 mL of 2% sodium chondroitin sulfate+1.6% sodium hyaluronic acid, respectively. On day 7, the animals were sacrificed and the bladders were removed for histopathological and immunohistochemical assessments.
Significant between-group differences were found in vascular congestion (P=0.006). The grade of submucosal edema in groups II and IV was significantly higher than in group I (P=0.006, P=0.006, respectively). In group I, the grade of granulation tissue was lower than the other 4 groups, but no significant difference was found between the remaining groups (P=0.016). Neutrophil cell infiltration was more intense in groups II and IV than in group I (P=0.006, P=0.006, respectively). Significant differences in the leukocyte and mast cell count were detected between groups II and IV (P<0.001, P<0.001, respectively). Abnormal zonula occludens-1 and uroplakin-III immunoreactivity in group II was higher than in groups I, III, or V (P=0.002, P=0.010, respectively). Interleukin-8 expression was lower in group V than in group II (P=0.001).
A single treatment of chondroitin sulfate and combined hyaluronic acid-chondroitin sulfate treatment demonstrated efficacy by suppressing inflammation and achieving improvements in the urothelium.
在间质性膀胱炎大鼠模型中比较三种膀胱内治疗药物：硫酸软骨素、透明质酸以及透明质酸 - 硫酸软骨素组合。
将35只雌性大鼠分为5组：对照组（I组）、等渗组（II组）、硫酸软骨素组（III组）、透明质酸组（IV组）和透明质酸 - 硫酸软骨素组（V组）。通过经尿道途径向所有实验组膀胱内灌注1 mL过氧化氢（H2O2）15分钟诱导化学性膀胱炎。在诱导化学性膀胱炎2天后，每隔一天进行一次治疗，共3个疗程。II组、III组、IV组和V组分别接受1 mL 0.9%氯化钠、1 mL 0.2%硫酸软骨素钠、1 mL低分子量透明质酸和1 mL 2%硫酸软骨素钠 + 1.6%透明质酸钠。在第7天，处死动物并取出膀胱进行组织病理学和免疫组织化学评估。
在血管充血方面发现组间存在显著差异（P = 0.006）。II组和IV组的黏膜下水肿程度显著高于I组（分别为P = 0.006，P = 0.006）。在I组中，肉芽组织等级低于其他4组，但其余组之间未发现显著差异（P = 0.016）。II组和IV组的中性粒细胞浸润比I组更强烈（分别为P = 0.006, P = 0.006）。在II组和IV组之间检测到白细胞和肥大细胞计数存在显著差异（分别为P < 0.001，P < 0.001）。II组中紧密连接蛋白 - 1和尿路上皮蛋白 - III的异常免疫反应性高于I组、III组或V组（分别为P = 0.002，P = 0.010）。V组中白细胞介素 - 8表达低于II组（P = 0.001）。
单次硫酸软骨素治疗以及透明质酸 - 硫酸软骨素联合治疗通过抑制炎症和改善尿路上皮显示出疗效。
