Department of Urology, Akdeniz University School of Medicine, Antalya, Turkey.
Department of Histology and Embryology, Akdeniz University School of Medicine, Antalya, Turkey.
Int Braz J Urol. 2018 Sep-Oct;44(5):1014-1022. doi: 10.1590/S1677-5538.IBJU.2017.0713.
To evaluate the effect of intravesical hyaluronic acid (HA) treatment on inflammatory cells and the severity of inflammation in an interstitial cystitis rat model created with hydrogen chloride (HCL) via immunohistochemical studies and myeloperoxidase activity for the first time in the literature.
A total of 30 adult female white Rattus Norvegicus rats were divided into 3 groups as the HCL group, hyaluronic acid treatment (HCL-HA) group and control group. Chemical cystitis was created by administering HCL(400 microL,10 mM) except control group. A single dose of intravesical HA(0.5 mL,0.8 mg/mL) was administered to the treatment group. The bladder tissues of all subjects were immunohistochemically stained. The cell surface markers were used to evaluate inflammatory cell infiltration. Mast cell activation and IL-6 was evaluated to assess the inflammation and severity of inflammation, respectively. Myeloperoxidase activity was measured as it shows neutrophil density. Statistical significance was accepted as P<0.05.
It was observed that there was rich monocyte, T lymphocyte, B lymphocyte, and Natural Killer cells infiltration and high IL-6 levels in the bladder tissue after the intravesical hydrogen chloride instillation, especially in the stroma layer(p<0.005). In the HCL-HA group, severity of inflammation had statistically significantly regressed to the levels of the control group(p<0.005). An increase was observed in the bladder myeloperoxidase activity of the HCL group compared to the other two groups(p<0.05).
Single dose intravesical hyluronic acid instillation reduces inflammatory cell infiltration and the severity of bladder inflammation in the rat model of bladder pain syndrome/interstitial cystitis.
通过免疫组织化学研究和髓过氧化物酶活性，首次在文献中评估膀胱内透明质酸（HA）治疗对盐酸（HCL）诱导的间质性膀胱炎大鼠模型中炎症细胞和炎症严重程度的影响。
将 30 只成年雌性白色 Rattus Norvegicus 大鼠分为 HCL 组、透明质酸治疗（HCL-HA）组和对照组 3 组。除对照组外，各组均给予 HCL（400 μL，10 mM）建立化学性膀胱炎。治疗组单次给予膀胱内透明质酸（0.5 mL，0.8 mg/mL）。对所有标本进行膀胱组织免疫组织化学染色。用细胞表面标志物评估炎症细胞浸润。评估肥大细胞活化和 IL-6 以分别评估炎症和炎症严重程度。髓过氧化物酶活性作为中性粒细胞密度的指标进行测量。统计学意义被接受为 P<0.05。
观察到膀胱内注入盐酸后，膀胱组织中有丰富的单核细胞、T 淋巴细胞、B 淋巴细胞和自然杀伤细胞浸润，IL-6 水平升高，尤其是在基质层（p<0.005）。在 HCL-HA 组，炎症严重程度与对照组相比有统计学显著改善（p<0.005）。与其他两组相比，HCL 组膀胱髓过氧化物酶活性增加（p<0.05）。
单次膀胱内透明质酸灌注可减少膀胱疼痛综合征/间质性膀胱炎大鼠模型中炎症细胞浸润和膀胱炎症严重程度。
