Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutrition Engineering, Wuhan Polytechnic University, Wuhan, China.
Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Sciences, Wuhan, China.
Innate Immun. 2021 Feb;27(2):170-183. doi: 10.1177/1753425920983869. Epub 2021 Jan 27.
Long non-coding RNAs (lncRNAs) are emerging as key regulators in inflammation. However, their functions and profiles in LPS-induced inflammation in pigs are largely unknown. In this study, we profiled global lncRNA and mRNA expression changes in PBMCs treated with LPS using the lncRNA-seq technique. In total 43 differentially expressed (DE) lncRNAs and 1082 DE mRNAs were identified in porcine PBMCs after LPS stimulation. Functional enrichment analysis on DE mRNAs indicated these genes were involved in inflammation-related signaling pathways, including cytokine-cytokine receptor interaction, TNF-α, NF-κB, Jak-STAT and TLR signaling pathways. In addition, co-expression network and function analysis identified the potential lncRNAs related to inflammatory response and immune response. The expressions of eight lncRNAs (ENSSSCT00000045208, ENSSSCT00000051636, ENSSSCT00000049770, ENSSSCT00000050966, ENSSSCT00000047491, ENSSSCT00000049750, ENSSSCT00000054262 and ENSSSCT00000044651) were validated in the LPS-treated PBMCs by quantitative real-time PCR (qRT-PCR). In LPS-challenged piglets, we identified that expression of three lncRNAs (ENSSSCT00000051636, ENSSSCT00000049770, and ENSSSCT00000047491) was significantly up-regulated in liver, spleen and jejunum tissues after LPS challenge, which indicated that these lncRNAs might be important regulators for inflammation. This study provides the first lncRNA and mRNA transcriptomic landscape of LPS-mediated changes in porcine PBMCs, which might provide potential insights into lncRNAs involved in regulating inflammation in pigs.
长链非编码 RNA（lncRNA）作为炎症反应的关键调节因子而受到关注。然而，它们在 LPS 诱导的猪炎症中的功能和特征在很大程度上仍是未知的。在本研究中，我们使用 lncRNA-seq 技术分析了 LPS 处理的猪 PBMC 中的 lncRNA 和 mRNA 表达谱。在 LPS 刺激后，猪 PBMC 中鉴定出 43 个差异表达（DE）lncRNA 和 1082 个 DE mRNA。对 DE mRNAs 的功能富集分析表明，这些基因参与了炎症相关的信号通路，包括细胞因子-细胞因子受体相互作用、TNF-α、NF-κB、Jak-STAT 和 TLR 信号通路。此外，共表达网络和功能分析确定了与炎症反应和免疫反应相关的潜在 lncRNA。8 个 lncRNA（ENSSSCT00000045208、ENSSSCT00000051636、ENSSSCT00000049770、ENSSSCT00000050966、ENSSSCT00000047491、ENSSSCT00000049750、ENSSSCT00000054262 和 ENSSSCT00000044651）的表达通过定量实时 PCR（qRT-PCR）在 LPS 处理的 PBMC 中得到验证。在 LPS 处理的仔猪中，我们发现 3 个 lncRNA（ENSSSCT00000051636、ENSSSCT00000049770 和 ENSSSCT00000047491）在 LPS 挑战后在肝脏、脾脏和空肠组织中的表达显著上调，这表明这些 lncRNA 可能是炎症反应的重要调节因子。本研究提供了 LPS 介导的猪 PBMC 变化的首个 lncRNA 和 mRNA 转录组图谱，这可能为调节猪炎症的 lncRNA 提供潜在的见解。
