Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Key Laboratory of Ministry of Health, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Beijing, China.
Sci Rep. 2020 Nov 12;10(1):19629. doi: 10.1038/s41598-020-76701-2.
The aim of this study was to elucidate the expression profile and the potential role of long non-coding RNA (LncRNA) in the peripheral blood mononuclear cells of primary Sjögren's syndrome (pSS) patients. RNA-seq technology was used to detect the differentially expressed LncRNAs and mRNAs between five age-and sex-matched paired pSS patients and healthy control PBMCs. The selected LncRNAs were detected in the validation study by RT-qPCR in 16 paired pSS patients and healthy controls. The GO, KEGG, co-localization, and co-expression analysis were performed to enrich the potential gene functions and pathways. In this study, 44 out of 1772 LncRNAs and 1034 out of 15,424 mRNAs were expressed differentially in the PBMCs of pSS patients. LINC00426, TPTEP1-202, CYTOR, NRIR, and BISPR were validated as aberrantly expressed, and these LncRNAs strongly correlated with disease activity of pSS. GO and KEGG pathway analysis revealed the significant enrichment of biological processes, cellular components, and molecular function of the up and down-regulated mRNAs, which were mainly concentrated in the immune response and immune system processes. Co-localization and co-expression analysis also revealed that differentially expressed LncRNAs in the PBMCs of pSS were strongly correlated to the mRNA functioning associated with immune response and cell metastasis. Numerous LncRNAs and mRNAs were found differentially expressed in the PBMCs of pSS patients, especially NRIR and BISPR; they interacted with the co-localized and co-expressed mRNAs, which might participate in the pathogenesis of pSS through the NF-κB, JAK-STAT, and other signaling pathways that regulate cell metastasis.
本研究旨在阐明长链非编码 RNA（LncRNA）在原发性干燥综合征（pSS）患者外周血单个核细胞中的表达谱及其潜在作用。采用 RNA-seq 技术检测 5 对年龄和性别匹配的 pSS 患者和健康对照 PBMC 之间差异表达的 LncRNAs 和 mRNAs。在验证研究中，通过 RT-qPCR 检测了 16 对 pSS 患者和健康对照的选定 LncRNAs。进行 GO、KEGG、共定位和共表达分析，以富集潜在的基因功能和途径。在这项研究中，1772 个 LncRNAs 中有 44 个，15424 个 mRNAs 中有 1034 个在 pSS 患者的 PBMCs 中表达差异。LINC00426、TPTEP1-202、CYTOR、NRIR 和 BISPR 被验证为异常表达，这些 LncRNAs 与 pSS 的疾病活动强烈相关。GO 和 KEGG 通路分析显示，上调和下调的 mRNAs 的生物学过程、细胞成分和分子功能显著富集，主要集中在免疫反应和免疫系统过程中。共定位和共表达分析还表明，pSS PBMC 中差异表达的 LncRNAs 与与免疫反应和细胞转移相关的 mRNA 功能密切相关。在 pSS 患者的 PBMC 中发现了大量差异表达的 LncRNAs 和 mRNAs，特别是 NRIR 和 BISPR；它们与共定位和共表达的 mRNAs 相互作用，可能通过调节细胞转移的 NF-κB、JAK-STAT 等信号通路参与 pSS 的发病机制。
