Zhu Wenbo, Liang Jiankai, Tan Jingyi, Guo Li, Cai Jing, Hu Jun, Yan Guangmei, Liu Yang, Zhang Jiayu, Song Deli, Dan Jia, Wong Chun-Wa, Su Xingwen, Qiu Pengxin, Lin Yuan
Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
Hum Gene Ther. 2021 Feb;32(3-4):158-165. doi: 10.1089/hum.2020.273.
Alphavirus M1 is a promising oncolytic virus for cancer therapy. Here, we constructed a fluorescent reporter virus for real-time visualization and quantification of M1 virus both and . The reporter-encoding M1 virus maintained the characteristics of parental virus in the aspects of structure, replication capacity, the feature to induce cytopathic cell death, and the property of tumor targeting. The fluorescence is positively correlated with virus replication both and . More importantly, the reporter can be stably expressed for at least 10 generations in a serial passage assay. In summary, we successfully constructed stable and authentic reporter viruses for studying M1 virus and provided a feasible technical route for gene modification of oncolytic virus M1.
甲病毒M1是一种用于癌症治疗的很有前景的溶瘤病毒。在此，我们构建了一种荧光报告病毒，用于在体内和体外对M1病毒进行实时可视化和定量分析。编码报告基因的M1病毒在结构、复制能力、诱导细胞病变性细胞死亡的特性以及肿瘤靶向特性等方面保持了亲本病毒的特征。荧光在体内和体外均与病毒复制呈正相关。更重要的是，在连续传代试验中，该报告基因可稳定表达至少10代。总之，我们成功构建了用于研究M1病毒的稳定且可靠的报告病毒，并为溶瘤病毒M1的基因改造提供了一条可行的技术路线。
