State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, China
State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, China.
Gut. 2022 Jan;71(1):78-87. doi: 10.1136/gutjnl-2020-323347. Epub 2021 Jan 27.
Effects of the diet-induced gut microbiota dysbiosis reach far beyond the gut. We aim to uncover the direct evidence involving the gut-testis axis in the aetiology of impaired spermatogenesis.
An excessive-energy diet-induced metabolic syndrome (MetS) sheep model was established. The testicular samples, host metabolomes and gut microbiome were analysed. Faecal microbiota transplantation (FMT) confirmed the linkage between gut microbiota and spermatogenesis.
We demonstrated that the number of arrested spermatogonia was markedly elevated by using 10× single-cell RNA-seq in the MetS model. Furthermore, through using metabolomics profiling and 16S rDNA-seq, we discovered that the absorption of vitamin A in the gut was abolished due to a notable reduction of bile acid levels, which was significantly associated with reduced abundance of . Notably, the abnormal metabolic effects of vitamin A were transferable to the testicular cells through the circulating blood, which contributed to abnormal spermatogenesis, as confirmed by FMT.
These findings define a starting point for linking the testicular function and regulation of gut microbiota via host metabolomes and will be of potential value for the treatment of male infertility in MetS.
饮食引起的肠道微生物失调的影响远远超出肠道。我们旨在揭示涉及肠道-睾丸轴在精子发生受损发病机制中的直接证据。
建立了一种过量能量饮食诱导的代谢综合征 (MetS) 绵羊模型。分析了睾丸样本、宿主代谢组和肠道微生物组。粪便微生物群移植 (FMT) 证实了肠道微生物群与精子发生之间的联系。
我们通过在 MetS 模型中使用 10×单细胞 RNA-seq 表明，精原细胞停滞的数量明显增加。此外，通过代谢组学分析和 16S rDNA-seq，我们发现由于胆汁酸水平的显著降低，肠道对维生素 A 的吸收被阻断，这与显著减少的 显著相关。值得注意的是，维生素 A 的异常代谢作用通过循环血液传递到睾丸细胞，导致异常精子发生，FMT 证实了这一点。
这些发现为通过宿主代谢组将睾丸功能和肠道微生物群的调节联系起来奠定了基础，这对于 MetS 中的男性不育治疗具有潜在价值。
