Bahadori Mohammad Hadi, Azari Zoleikha, Zaminy Arash, Dabirian Sara, Mehrdad Seyed Mojtaba, Kondori Bahman Jalali
Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
Anat Cell Biol. 2021 Mar 31;54(1):104-111. doi: 10.5115/acb.20.228.
Papillary thyroid carcinoma (PTC) is one of the most common cancers of the endocrine system. Previous studies have shown that the extract of hull-less pumpkin seed (HLPS) has a significant anti-cancer effect. The aim of this study was to evaluate the effect of this plant extract on the proliferation of PTC cells. In this study, an extract of this plant was prepared by soxhlet extraction method and analyzed by Gas Chromatography-Mass Spectrometry. The cytotoxicity of PTX and plant extract was investigated using the methylthiazol tetrazolium (MTT) method. For careful investigation of morphological alteration, we used hematoxylin and eosin and Giemsa stinging. Based on MTT assay test, the IC value of paclitaxel (PTX) was significantly less than the hydro-alcoholic extract of HLPS at all of the incubation time. Our results of histological staining showed that HLPS and PTX induced significant morphological alteration in the PTC cultured cell that consistent with cell death. Comparing the groups treated by PTX or HLPS with control group showed significant differences. It seems that HLPS extract has an apparent effect on treatment of PTC, at least in laboratory condition, albeit for realistic decision about the effect of HLPS on PTC, more molecular investigations are necessary.
甲状腺乳头状癌（PTC）是内分泌系统最常见的癌症之一。先前的研究表明，无壳南瓜籽提取物（HLPS）具有显著的抗癌作用。本研究的目的是评估这种植物提取物对PTC细胞增殖的影响。在本研究中，采用索氏提取法制备该植物提取物，并通过气相色谱 - 质谱联用仪进行分析。使用甲基噻唑四氮唑（MTT）法研究紫杉醇（PTX）和植物提取物的细胞毒性。为了仔细研究形态学改变，我们使用苏木精和伊红以及吉姆萨染色。基于MTT试验，在所有孵育时间，紫杉醇（PTX）的IC值均显著低于HLPS的水醇提取物。我们的组织学染色结果表明，HLPS和PTX在PTC培养细胞中诱导了与细胞死亡一致的显著形态学改变。将PTX或HLPS处理组与对照组进行比较显示出显著差异。似乎HLPS提取物对PTC的治疗有明显作用，至少在实验室条件下如此，尽管要对HLPS对PTC的作用做出实际决策，还需要更多的分子研究。
