Let-7i-5p通过靶向CKIP-1发挥假定的成骨分化促进因子的作用。
Let-7i-5p functions as a putative osteogenic differentiation promoter by targeting CKIP-1.
作者信息
Zhang Yang, Cheng Wei, Han Biao, Guo Yong, Wei Shuping, Yu Lu, Zhang Xizheng
机构信息
The School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 China.
Tianjin Medical University General Hospital, Tianjin, 300052 China.
出版信息
Cytotechnology. 2021 Feb;73(1):79-90. doi: 10.1007/s10616-020-00444-1. Epub 2021 Jan 4.
UNLABELLED
MicroRNA (miRNA) is an endogenous regulatory small molecule RNA. Growing evidence shows that miRNA plays an important regulatory role in gene expression. Although miRNA is a more intensive regulatory noncoding RNA in recent years, few studies have investigated the regulation of targeting genes involved in bone repair. Meanwhile, as a negative bone regulator, previous studies showed that casein kinase 2-interacting protein 1 (CKIP-1) is closely associated with bone formation and regeneration. However, the gene knockout method may not be suitable for clinical application. Therefore, it was hypothesized that miRNA molecules can inhibit the expression of CKIP-1 and ultimately promote the osteogenesis process. The present study revealed that let-7i-5p plays an important role in the process of fracture healing by inhibiting the expression of CKIP-1. Related research provides a novel gene target for fracture healing.
SUPPLEMENTARY INFORMATION
The online version of this article (10.1007/s10616-020-00444-1) contains supplementary material, which is available to authorized users.
未标注
微小RNA（miRNA）是一种内源性调节小分子RNA。越来越多的证据表明，miRNA在基因表达中起重要调节作用。尽管近年来miRNA是一种更强效的调节性非编码RNA，但很少有研究探讨其对参与骨修复的靶基因的调节作用。同时，作为一种负性骨调节因子，先前的研究表明酪蛋白激酶2相互作用蛋白1（CKIP-1）与骨形成和再生密切相关。然而，基因敲除方法可能不适用于临床应用。因此，推测miRNA分子可抑制CKIP-1的表达并最终促进成骨过程。本研究表明，let-7i-5p通过抑制CKIP-1的表达在骨折愈合过程中起重要作用。相关研究为骨折愈合提供了一个新的基因靶点。
补充信息
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