Department of Physiology, Faculty of Medicine, University of Niš, Zorana Đinđića, 81 Niš, Serbia.
Department of Pharmacy, Faculty of Medicine, University of Niš, Zorana Đinđića, 81 Niš, Serbia.
Oxid Med Cell Longev. 2021 Jan 7;2021:6635310. doi: 10.1155/2021/6635310. eCollection 2021.
Both oxidative and psychological (mental) stress are the likely culprits for several acute and chronic health disturbances, and adequate tests mimicking that are needed. Herein, in controlled laboratory surroundings, a PEBL (Psychology Experiment Building Language) test battery was used to evoke stress-related biological responses followed by tracking changes in saliva parameters. The study objectives were to determine the impact of psychological stress on selected salivatory parameters and to assess the correlation between the determined oxidative and stress parameters. The study was conducted on 36 healthy young subjects, mainly females ( = 24). Before and following the completion of a battery of four PEBL tests, subjects' saliva samples were collected. Stress-evoking changes in total antioxidant capacity and nitrite/nitrate levels, as oxidative stress parameters, and cortisol and immunoglobulin A (IgA), as parameters of psychological stress, were established and mutually correlated by comparing the values of the evaluated parameters pre- and post-PEBL test. The results showed that there is no change in the total salivary antioxidant capacity ( > 0.05); however, there was a significant increase in nitrites/nitrates levels after the PEBL test ( = 0.007). On the other hand, the determined cortisol levels after the test battery were found to be statistically significantly increased ( = 0.025) when compared to the values obtained before the test, while the levels of IgA were found to be statistically significantly decreased ( < 0.001). The only statistically significant correlation between the changes in the studied parameters was found to be the one between cortisol and IgA levels (Spearman's Rö = -0.4). These results suggest that the short-term stress induced by the PEBL test does evoke changes in the salivary mental stress-related parameters (an increase in cortisol and nitrite/nitrate levels, and a decrease in IgA), but not in the total antioxidant capacity. They also indicate that the constructed PEBL four-test battery might represent an adequate laboratory stress-inducing paradigm.
氧化应激和心理应激（精神应激）都可能是多种急性和慢性健康紊乱的罪魁祸首，因此需要进行适当的模拟测试。在此，我们在受控的实验室环境中使用 PEBL（心理学实验构建语言）测试组来引发与应激相关的生物学反应，然后跟踪唾液参数的变化。本研究的目的是确定心理应激对选定唾液参数的影响，并评估所确定的氧化应激和应激参数之间的相关性。本研究在 36 名健康的年轻受试者（主要为女性，n=24）中进行。在完成四组 PEBL 测试前后，收集了受试者的唾液样本。通过比较评估参数的测试前后值，确定了氧化应激参数（总抗氧化能力和亚硝酸盐/硝酸盐水平）和心理应激参数（皮质醇和免疫球蛋白 A（IgA））的应激诱导变化，并相互关联。结果表明，总唾液抗氧化能力没有变化（>0.05）；然而，在 PEBL 测试后，硝酸盐/硝酸盐水平显著增加（=0.007）。另一方面，与测试前相比，测试后确定的皮质醇水平发现有统计学显著增加（=0.025），而 IgA 水平发现有统计学显著降低（<0.001）。在所研究参数的变化之间仅发现一个具有统计学显著相关性，即皮质醇和 IgA 水平之间的相关性（Spearman 的 Rö=-0.4）。这些结果表明，PEBL 测试引起的短期应激确实会引起唾液与精神应激相关的参数发生变化（皮质醇和硝酸盐/硝酸盐水平增加，IgA 水平降低），但不会引起总抗氧化能力发生变化。它们还表明，构建的 PEBL 四测试组可能代表一种适当的实验室应激诱导范式。
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