Lopa Simin Shabnam, Al-Amin Md Yusuf, Hasan Md Kamrul, Ahammed Md Salim, Islam Km Monirul, Alam A H M Khurshid, Tanaka Toshihisa, Sadik Md Golam
Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
Department of Psychiatry, Osaka University Graduate School of Medicine, Osaka, Japan.
Int J Food Sci. 2021 Jan 11;2021:8862025. doi: 10.1155/2021/8862025. eCollection 2021.
, a popular vegetable in Bangladesh, is used in folk medicine to treat diseases of the nervous system. The objective of this study was to investigate the phytochemical profile and cholinesterase inhibitory and antioxidant potential of the extracts of . . Among the four tested extracts, the chloroform extract was found to exert the highest inhibition against both the acetylcholinesterase and butyrylcholinesterase enzymes with the IC (concentration required for 50% inhibition) values of 83.90 g/mL and 48.14 g/mL, respectively. Likewise, the chloroform extract showed the highest radical scavenging activity and reducing power. In DPPH radical scavenging assay, the IC value was found to be 113.27 g/mL, and in reducing power assay, the absorbance was found to be 1.916 at a concentration of 50 g/mL. Phytochemical analyses revealed that the chloroform extract contained 19.16 mg gallic acid equivalent (GAE)/g extract of phenolics and 41.84 mg catechin equivalent (CE)/g extract of flavonoids, which appeared to be the highest among the extracts. A significant correlation was observed between phenolic content and butyrylcholinesterase inhibition and antioxidant activity, while a moderate correlation was seen between flavonoid content and cholinesterase inhibition and antioxidant activity. These findings suggest that . is a natural source of cholinesterase inhibitors and antioxidants, which could be utilized as functional foods for Alzheimer's disease management.
在孟加拉国是一种受欢迎的蔬菜，在民间医学中用于治疗神经系统疾病。本研究的目的是调查……提取物的植物化学特征、胆碱酯酶抑制和抗氧化潜力。在四种测试提取物中，氯仿提取物对乙酰胆碱酯酶和丁酰胆碱酯酶的抑制作用最强，其IC（50%抑制所需浓度）值分别为83.90μg/mL和48.14μg/mL。同样，氯仿提取物表现出最高的自由基清除活性和还原能力。在DPPH自由基清除试验中，IC值为113.27μg/mL，在还原能力试验中，浓度为50μg/mL时吸光度为1.916。植物化学分析表明，氯仿提取物含有19.16mg没食子酸当量（GAE）/g提取物的酚类物质和41.84mg儿茶素当量（CE）/g提取物的黄酮类化合物，这似乎是提取物中最高的。酚类含量与丁酰胆碱酯酶抑制和抗氧化活性之间存在显著相关性，而黄酮类含量与胆碱酯酶抑制和抗氧化活性之间存在中等相关性。这些发现表明……是胆碱酯酶抑制剂和抗氧化剂的天然来源，可作为治疗阿尔茨海默病的功能性食品。
