Department of Respiratory and Critical Medicine, The Second Affiliated Hospital of Harbin Medical University, China.
Department of Respiratory Medicine, Harbin Chest Hospital, China.
Biomed Res Int. 2021 Jan 9;2021:8888280. doi: 10.1155/2021/8888280. eCollection 2021.
The present study focused on the potential clinical significance of miR-3934 in the occurrence and development of asthma.
80 asthma and 80 healthy controls were recruited in this study. The peripheral blood mononuclear cells (PBMCs) and serum samples of the asthma patients as well as the healthy controls were isolated, and the expression levels of miR-3934 in PBMCs were examined by RT-qPCR methods. Furthermore, the relationship between the level of miR-3934 in PBMCs and the disease severity has been analyzed, and the potential diagnostic value of miR-3934 was evaluated by the receiver operating characteristics (ROC) curve. Finally, the expression level of IL-6, IL-8, and IL-33 have been detected using the ELISA kits, and Pearson's correlation analysis was performed to investigate the relationship between the level of miR-3934 in PBMCs and the serum expression of those inflammatory cytokines in asthma patients.
miR-3934 was dramatically decreased in PBMCs of the asthma patients, and miR-3934 was markedly reduced in PBMCs of patients with severe asthma vs. mild asthma. Furthermore, ROC analysis showed that levels of miR-3934 in PBMCs can distinguish asthma patient, especially the severe asthma patients from the controls. Finally, the levels of miR-3934 in PBMCs were negatively correlated with the serum levels of IL-6, IL-8, and IL-33 in asthma patients, respectively.
miR-3934 was downregulated in PBMCs of asthmatic patients and may function as a potential diagnosis biomarker.
本研究旨在探讨 miR-3934 在哮喘发生发展中的潜在临床意义。
本研究纳入 80 例哮喘患者和 80 例健康对照者。分离哮喘患者和健康对照者的外周血单个核细胞（PBMCs）和血清样本，采用 RT-qPCR 方法检测 PBMCs 中 miR-3934 的表达水平。分析 miR-3934 水平与疾病严重程度的关系，并通过受试者工作特征（ROC）曲线评估 miR-3934 的潜在诊断价值。最后，采用 ELISA 试剂盒检测 IL-6、IL-8 和 IL-33 的表达水平，采用 Pearson 相关分析探讨 PBMCs 中 miR-3934 水平与哮喘患者血清中这些炎症细胞因子表达的关系。
哮喘患者 PBMCs 中 miR-3934 表达显著降低，重度哮喘患者 PBMCs 中 miR-3934 表达显著低于轻度哮喘患者。ROC 分析表明，PBMCs 中 miR-3934 水平可区分哮喘患者，尤其是重度哮喘患者与健康对照者。最后，PBMCs 中 miR-3934 水平与哮喘患者血清中 IL-6、IL-8 和 IL-33 水平呈负相关。
miR-3934 在哮喘患者 PBMCs 中下调，可能作为潜在的诊断生物标志物。
