Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC-CONICET), Fundación Para Investigaciones Biológicas Aplicadas (FIBA), Vieytes 3103, 7600, Mar del Plata, Argentina.
Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (FCEyN, UNMdP), Funes 3250, 7600, Mar del Plata, Argentina.
Planta. 2021 Jan 27;253(2):50. doi: 10.1007/s00425-021-03575-7.
Morpho-physiological changes were observed in Arabidopsis plants acclimated to long-term combined cold and water deficit stresses. Limiting growth and differences in bolting, flowering, and silique development were evidenced. In nature, plants are exposed to multiple and simultaneous abiotic stresses that influence their growth, development, and reproduction. In the last years, the study of combined stresses has aroused the interest to know the physiological and molecular responses, because these new stress conditions are probed to be different from the sum of the individual stress. We are interested in the study of the acclimation of plants growing under the combination of cold and water deficit stresses prevalent in cold-arid or semi-arid climates worldwide. We hypothesized that the reproduction of the acclimated plants will be compromised and affected. Arabidopsis plants were submitted to long-term combined stress from the beginning to the reproductive stage, when floral bud was visible, until the silique development. Our results demonstrate severe morpho-anatomical changes after acclimation to combined stress. Inflorescence stem morphology was altered having a delayed bolting and a limited growth. Flowering and silique formation were delayed, and a higher size in the corolla and the petals was observed. Flower and silique number were severely diminished as a result of combined stress, unlike acclimated plants to individual cold stress. These traits were recovered after deacclimation to optimal conditions and plants achieved similar silique production as control plants. The long-term stress results suggest that there is not a single dominant stress, but there is an alternating dominance depending on the structure or the plant stage development evaluated.
在适应长期冷胁迫和水分亏缺复合胁迫的拟南芥植株中观察到形态生理变化。证明了生长受限以及抽薹、开花和角果发育的差异。在自然界中，植物会同时受到多种非生物胁迫的影响，这些胁迫会影响它们的生长、发育和繁殖。在过去的几年中，对复合胁迫的研究引起了人们的兴趣，以了解生理和分子反应，因为这些新的胁迫条件与单个胁迫的总和不同。我们对研究在世界范围内寒冷干旱或半干旱气候下普遍存在的冷胁迫和水分亏缺胁迫组合下生长的植物的适应感兴趣。我们假设适应这些胁迫的植物的繁殖能力会受到损害和影响。拟南芥植物从开始到生殖阶段（可见花芽时），直到角果发育，都经历了长期的复合胁迫。我们的结果表明，适应复合胁迫后会发生严重的形态解剖变化。花序茎形态发生改变，抽薹延迟，生长受限。开花和角果形成延迟，观察到花冠和花瓣尺寸增大。由于复合胁迫，花和角果的数量严重减少，与适应单一冷胁迫的植物不同。在解除适应最优条件后，这些特性得到恢复，植物的角果产量与对照植物相似。长期胁迫的结果表明，没有单一的主导胁迫，而是存在交替的主导胁迫，这取决于所评估的结构或植物发育阶段。
