MS Proteomics Research Group, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Budapest, 1117, Hungary.
Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Budapest, 1111, Hungary.
Anal Bioanal Chem. 2021 Mar;413(7):1779-1785. doi: 10.1007/s00216-021-03152-7. Epub 2021 Jan 27.
Chondroitin sulfate (CS) is a widely studied class of glycosaminoglycans, responsible for diverse biological functions. Structural analysis of CS is generally based on disaccharide analysis. Sample preparation is a key analytical issue in this case. However, a detailed study on the stability and recovery of CS-derived species has been lacking so far. We have found that for solvent exchange, in general, vacuum evaporation (SpeedVac) is much preferable than lyophilization. Moreover, in the case of aqueous solutions, higher recovery was experienced than in solutions with high organic solvent content. Storage of the resulting disaccharide mixture in typical HPLC injection solvents is also critical; decomposition starts after 12 h at 4 °C; therefore, the mixtures should not be kept in the sample tray of an automatic injector for a long time. The study, therefore, lays down suggestions on proper sample preparation and measurement conditions for biologically derived chondroitin sulfate species.
硫酸软骨素（CS）是一类广泛研究的糖胺聚糖，负责多种生物学功能。CS 的结构分析通常基于二糖分析。在这种情况下，样品制备是一个关键的分析问题。然而，到目前为止，还缺乏对 CS 衍生物种的稳定性和回收率的详细研究。我们发现，对于溶剂交换，一般来说，真空蒸发（SpeedVac）比冻干更可取。此外，在水溶液的情况下，与高有机溶剂含量的溶液相比，回收率更高。所得二糖混合物在典型的 HPLC 进样溶剂中的储存也很关键；在 4°C 下 12 小时后开始分解；因此，混合物不应在自动进样器的样品托盘上长时间保存。因此，该研究为生物衍生的硫酸软骨素物种的适当样品制备和测量条件提出了建议。
