MS Proteomics Research Group, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Budapest, Hungary.
Methods Mol Biol. 2023;2619:71-90. doi: 10.1007/978-1-0716-2946-8_6.
Glycosaminoglycans (GAGs) are built up of repeating disaccharide units resulting in long, linear polysaccharide chains. In most classes of GAGs, sulfation and epimerization complicate the structure of the chain and influence biochemical functions. The most widespread way of their investigation by instrumental analytical techniques is to degrade them into the constituent disaccharide building blocks, followed by capillary electrophoresis or high-performance liquid chromatography (HPLC) separation. The analysis of GAG disaccharides with varying sulfation degrees poses a real challenge both from chromatographic and mass spectrometric (MS) points of view. This necessitates the constant improvement of their analytical methodology. In this chapter, an optimized workflow will be discussed for the sample preparation and subsequent HPLC-MS characterization of tissue-derived chondroitin sulfate and heparan sulfate.
糖胺聚糖（GAGs）由重复的二糖单元构成，形成长的线性多糖链。在大多数 GAG 类别中，硫酸化和差向异构化使链的结构复杂化，并影响生物化学功能。用仪器分析技术对 GAG 进行研究的最常见方法是将其降解为组成二糖结构单元，然后进行毛细管电泳或高效液相色谱（HPLC）分离。分析具有不同硫酸化程度的 GAG 二糖从色谱和质谱（MS）的角度来看都是一个真正的挑战。这需要不断改进它们的分析方法。在本章中，将讨论用于组织来源的硫酸软骨素和硫酸乙酰肝素的样品制备和随后的 HPLC-MS 表征的优化工作流程。
