Department of Respiratory and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan. China.
Eur Rev Med Pharmacol Sci. 2021 Jan;25(1):208-214. doi: 10.26355/eurrev_202101_24386.
To detect the expressions of tumor necrosis factor-α-induced protein-8-like 2 (TIPE2) and tissue factor (TF) in peripheral blood mononuclear cells (PBMCs) of patients with acute exacerbation of bronchial asthma (BA), and to analyze their associations with changes in inflammatory factors and T lymphocytes.
A total of 59 patients with BA treated in our hospital from February 2018 to April 2019 were selected as objects, including 30 cases in the acute exacerbation phase (BA group) and 29 in the remission phase (RE group). During the same period, 28 people receiving physical examinations were selected as healthy controls (Control group). The proportion of eosinophils in the sputum and the fractional exhaled nitric oxide (FeNO) level were detected in each subject. Blood samples were collected in patients of BA group and Control group, aiming to isolate PBMCs. Then, the messenger RNA (mRNA) expressions of TIPE2 and TF in PBMCs were determined via reverse transcription-polymerase chain reaction (RT-PCR). Serum levels of interleukin-1β (IL-1β) and IL-6 in BA group and Control group were determined via enzyme-linked immunosorbent assay (ELISA), and protein expressions of serum T helper 1 (Th1) and Th2 cells in BA group and Control group were detected using Western blotting.
Compared with those in Control group, the proportion of eosinophils and FeNO level increased in BA and RE group, which were more pronounced in BA group. Downregulated mRNA level of TIPE2, and upregulated TF were detected in BA and RE groups compared to those of Control group, and the expression changes were more significant in the former group. Enzyme-linked immunosorbent assay (ELISA) data showed that serum levels of IL-1β and IL-6 were significantly elevated in BA and RE groups in comparison to Control group, especially BA group. In addition, protein level of Th1 cells was downregulated, while that of Th2 was upregulated in BA group and RE group compared to those of Control group, and a more significant change was observed in BA group compared to that of RE group.
In patients with acute exacerbation of BA, the expression of TIPE2 in PBMCs declined, while that of TF rose, which were negatively correlated with each other. Moreover, the proportion of Th1 cells declined in patients with acute exacerbation of BA, indicating that it is associated with the lung function, inflammatory level and proportion of eosinophils.
检测肿瘤坏死因子-α诱导蛋白 8 样蛋白 2（TIPE2）和组织因子（TF）在外周血单个核细胞（PBMCs）中的表达，分析其与炎症因子和 T 淋巴细胞变化的关系。
选择 2018 年 2 月至 2019 年 4 月在我院治疗的 59 例支气管哮喘急性加重期（BA）患者为研究对象，包括急性加重期 30 例（BA 组），缓解期 29 例（RE 组）。同期选择 28 名体检者为健康对照组（对照组）。检测各组受试者痰中嗜酸性粒细胞比例和呼出气一氧化氮（FeNO）水平。BA 组和对照组采集血样，分离 PBMCs，采用逆转录-聚合酶链反应（RT-PCR）法检测 PBMCs 中 TIPE2 和 TF 的信使 RNA（mRNA）表达。采用酶联免疫吸附试验（ELISA）法检测 BA 组和对照组血清白细胞介素-1β（IL-1β）和 IL-6 水平，采用 Western blot 法检测 BA 组和对照组血清辅助性 T 细胞 1（Th1）和 Th2 细胞的蛋白表达。
与对照组相比，BA 组和 RE 组的嗜酸性粒细胞比例和 FeNO 水平升高，BA 组更为明显。BA 组和 RE 组的 TIPE2mRNA 水平下调，TF 表达上调，且前者的表达变化更为显著。ELISA 数据显示，BA 组和 RE 组血清中 IL-1β和 IL-6 水平均明显高于对照组，尤以 BA 组更为显著。此外，与对照组相比，BA 组和 RE 组 Th1 细胞蛋白水平下调，Th2 细胞蛋白水平上调，且 BA 组的变化更为显著。
在支气管哮喘急性加重期患者中，PBMCs 中 TIPE2 的表达下降，TF 的表达升高，二者呈负相关。此外，急性加重期支气管哮喘患者 Th1 细胞比例下降，与肺功能、炎症水平和嗜酸性粒细胞比例有关。
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