miR-185-5p 的检测有助于反映 Th1/Th2 细胞失衡、炎症细胞因子产生和儿童哮喘恶化风险。
MiR-185-5p measurement assists in reflecting Th1/Th2 cell imbalance, inflammatory cytokine production, and exacerbation risk for childhood asthma.
机构信息
Tianjin Medical University, Tianjin, China.
Department of Pediatric, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China.
出版信息
Allergol Immunopathol (Madr). 2023 May 1;51(3):91-98. doi: 10.15586/aei.v51i3.810. eCollection 2023.
BACKGROUND
MicroRNA (miR)-185-5p participates in the pathology of asthma by regulating immune imbalance, inflammation, periostin synthesis, and smooth muscle contraction. This study intended to explore the dysregulation of miR-185p and its correlation with T-helper (Th)1, Th2 cells, and inflammatory cytokines in childhood asthma.
METHODS
In 150 childhood asthma patients and 30 healthy controls (HCs), miR-185-5p from peripheral blood mononuclear cells was detected using reverse transcription-quantitative polymerase chain reaction, Th cells from peripheral blood samples were detected using flow cytometry, inflammatory cytokines from serum samples were detected using enzyme-linked immunosorbent assay.
RESULTS
MiR-185-5p was increased in childhood asthma patients versus HCs [median (interquartile range (IQR)): 2.315 (1.770-3.855) versus 1.005 (0.655-1.520)] ( < 0.001). Meanwhile, miR-185-5p was negatively associated with Th1 cells ( = 0.035) but positively correlated with Th2 cells ( = 0.006) and IL-4 ( = 0.003) in childhood asthma patients; however, miR-185-5p was not linked to Th1 cells, Th2 cells, IFN-γ, or IL-4 in HCs (all > 0.05). In addition, miR-185-5p was positively related to TNF-α ( < 0.001), IL-1β ( = 0.015), and IL-6 ( = 0.008) in childhood asthma patients, miR-185-5p was only linked to TNF-α ( = 0.040) but not IL-1β or IL-6 (both > 0.05) in HCs. Moreover, miR-185-5p was increased in exacerbated childhood asthma patients versus remissive patients [median (IQR): 3.170 (2.070-4.905) versus 1.900 (1.525-2.615)] ( < 0.001). Besides, miR-185-5p was highest in patients with severe exacerbation followed by patients with moderate exacerbation, and lowest in patients with mild exacerbation ( = 0.010).
CONCLUSION
MiR-185-5p is associated with imbalanced Th1/Th2 cells, increased inflammatory cytokines along with elevated exacerbation risk, and severity in childhood asthma patients.
背景
微小 RNA（miR）-185-5p 通过调节免疫失衡、炎症、骨膜蛋白合成和平滑肌收缩参与哮喘的病理过程。本研究旨在探讨 miR-185p 的失调及其与儿童哮喘中 Th1、Th2 细胞和炎症细胞因子的相关性。
方法
在 150 名儿童哮喘患者和 30 名健康对照者（HCs）中，采用逆转录定量聚合酶链反应检测外周血单个核细胞中的 miR-185-5p，采用流式细胞术检测外周血中的 Th 细胞，采用酶联免疫吸附试验检测血清中的炎症细胞因子。
结果
与 HCs 相比，儿童哮喘患者的 miR-185-5p 升高[中位数（四分位距（IQR））：2.315（1.770-3.855）比 1.005（0.655-1.520）]（<0.001）。同时，miR-185-5p 与儿童哮喘患者中的 Th1 细胞呈负相关（=0.035），与 Th2 细胞和 IL-4 呈正相关（=0.006 和 0.003）；然而，miR-185-5p 与 HCs 中的 Th1 细胞、Th2 细胞、IFN-γ 或 IL-4 均无相关性（均>0.05）。此外，miR-185-5p 与儿童哮喘患者中的 TNF-α（<0.001）、IL-1β（=0.015）和 IL-6（=0.008）呈正相关，miR-185-5p 仅与 TNF-α（=0.040）相关，而与 IL-1β 或 IL-6 均不相关（均>0.05）。此外，与缓解期儿童哮喘患者相比，恶化期儿童哮喘患者的 miR-185-5p 升高[中位数（IQR）：3.170（2.070-4.905）比 1.900（1.525-2.615）]（<0.001）。此外，miR-185-5p 在重度恶化患者中最高，在中度恶化患者中次之，在轻度恶化患者中最低（=0.010）。
结论
miR-185-5p 与 Th1/Th2 细胞失衡、炎症细胞因子水平升高以及儿童哮喘患者恶化和严重程度相关。
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