Department of Cardiac Surgery, Huai'an First People's Hospital, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, China.
Eur Rev Med Pharmacol Sci. 2021 Jan;25(1):287-292. doi: 10.26355/eurrev_202101_24394.
Changes of microRNAs (miRNAs) may contribute to the pathogenesis and progression of postoperative atrial fibrillation (POAF) in patients undergoing cardiac valve surgery. This study aimed to measure the expression levels of miRNAs in peripheral blood, as well as their target mRNAs, in POAF patients and normal controls (non-POAF), and to evaluate the potential of miRNAs as promising biomarkers to predict POAF.
The expression of miRNAs in peripheral blood, including miR-27b, miR-133a, miR-328, miR-499 and their target mRNAs, was analyzed in 109 POAF patients and 96 non-POAF patients via quantitative real-time polymerase chain reaction (RT-PCR). We compared differences between the two groups and also analyzed the treat reaction to amiodarone.
All miRNAs in POAF patients were significantly highly expressed. Compared to non-POAF, the expression of miR-27b, miR-133a, miR-328, miR-499 increased in both groups of POAF patients, and miR-499 was the only upregulated miRNAs in the amiodarone - group versus amiodarone + group and non-POAF. Among the upregulated miRNAs, miR-499 expression significantly changed in amiodarone + and amiodarone - patients (p = 0.005). The ROC curve analysis revealed that miR-499 might be a potential therapeutic response biomarker. The miRNA-mRNA interactions revealed 10 mRNAs regulated by miR-27b, miR-133a, and miR- 499.
We found an expression on miR-133a, miR-27b, miR-328, and miR-499 was significantly different between these groups, with a high expression being observed in POAF patients compared to non-POAF patients. Further, the present results showed that miR-499 was significantly upregulated in amiodarone - patients, compared to non-POAF, and amiodarone + patients. This finding indicates that miR-499 may be a potential biomarker for predicting the occurrence of POAF after cardiac valve surgery and treat the reaction to amiodarone.
微小 RNA（miRNA）的变化可能导致心脏瓣膜手术后并发心房颤动（POAF）的发生和发展。本研究旨在测量 POAF 患者和正常对照组（非 POAF）外周血中 miRNA 的表达水平及其靶 mRNA，并评估 miRNA 作为预测 POAF 的有前途的生物标志物的潜力。
通过实时定量聚合酶链反应（RT-PCR）分析了 109 例 POAF 患者和 96 例非 POAF 患者外周血中 miRNA 的表达，包括 miR-27b、miR-133a、miR-328、miR-499 及其靶 mRNA。我们比较了两组之间的差异，并分析了胺碘酮的治疗反应。
POAF 患者的所有 miRNA 均显著高表达。与非 POAF 相比，POAF 患者的 miR-27b、miR-133a、miR-328、miR-499 表达均升高，而 miR-499 是胺碘酮组与胺碘酮+组和非 POAF 中唯一上调的 miRNA。在上调的 miRNA 中，miR-499 在胺碘酮+和胺碘酮-患者中的表达显著变化（p=0.005）。ROC 曲线分析显示，miR-499 可能是一种潜在的治疗反应生物标志物。miRNA-mRNA 相互作用揭示了 10 个受 miR-27b、miR-133a 和 miR-499 调控的 mRNA。
我们发现 miR-133a、miR-27b、miR-328 和 miR-499 在这些组之间的表达明显不同，POAF 患者的表达明显高于非 POAF 患者。此外，本研究结果表明，与非 POAF 相比，胺碘酮-患者的 miR-499 表达显著上调，而胺碘酮+患者的 miR-499 表达显著上调。这一发现表明，miR-499 可能是预测心脏瓣膜手术后 POAF 发生和胺碘酮治疗反应的潜在生物标志物。
