循环外泌体来源的 miR-122-5p 是预测术后心房颤动的新型生物标志物。
Circulating exosome-derived miR-122-5p is a novel biomarker for prediction of postoperative atrial fibrillation.
机构信息
Department of Cardiovascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China.
School of Nursing, Capital Medical University, Beijing, 100069, China.
出版信息
J Cardiovasc Transl Res. 2022 Dec;15(6):1393-1405. doi: 10.1007/s12265-022-10267-4. Epub 2022 May 5.
Postoperative atrial fibrillation (POAF) is a frequent complication associated with increased periprocedural mortality and morbidity after cardiac surgery. Our study aimed to identify the difference in exosomal miRNA and further explore its role in the diagnosis of POAF. First, the differentially expressed miRNAs (DEMs) were obtained by high-throughput RNA sequencing. Second, the DEMs target genes were put into gene ontology (GO) and KEGG pathway analysis. Third, real-time quantification PCR (RT-qPCR) was used to verify the DEMs. Finally, we revealed 23 DEMs in POAF patients. Furthermore, analysis of gene function revealed that DEMs may affect atrial structure through many signaling pathways. We also found that miR-122-5p was up-regulated in POAF patients, but there are no significant changes in miR-191-5p, miR-181a-5p, miR-155-5p and miR-151a-5p. Our study revealed that exosomal miRNAs exert enormous potential in evaluating the severity or prognostic of POAF.
术后心房颤动（POAF）是心脏手术后围手术期死亡率和发病率增加的常见并发症。我们的研究旨在确定外泌体 miRNA 的差异，并进一步探讨其在 POAF 诊断中的作用。首先，通过高通量 RNA 测序获得差异表达 miRNA（DEM）。其次，将 DEMs 靶基因进行基因本体（GO）和 KEGG 通路分析。第三，采用实时定量 PCR（RT-qPCR）验证 DEM。最后，我们在 POAF 患者中发现了 23 个 DEM。此外，基因功能分析表明，DEM 可能通过多种信号通路影响心房结构。我们还发现，miR-122-5p 在 POAF 患者中上调，但 miR-191-5p、miR-181a-5p、miR-155-5p 和 miR-151a-5p 没有明显变化。我们的研究表明，外泌体 miRNA 在评估 POAF 的严重程度或预后方面具有巨大的潜力。
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