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利用水热法合成的氧化锌纳米粒子对番茄中的 进行管理。

Management of in Tomato Using ZnO Nanoparticles Synthesized Through .

机构信息

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Department of Plant Pathology, The University of Agriculture Peshawar, Peshawar 2500, Pakistan.

出版信息

Plant Dis. 2021 Oct;105(10):3224-3230. doi: 10.1094/PDIS-08-20-1763-RE. Epub 2021 Oct 26.

Abstract

flower extract was used as a biocompatible material for synthesis of zinc oxide nanoparticles (ZnONPs). The synthesized NPs were evaluated for their antibacterial potential in vitro and in vivo against the Gram-negative bacterium , which causes devastating bacterial wilt disease in tomato and other crops. Synthesized ZnONPs were further analyzed by UV-visible spectroscopy, Fourier transform infrared spectroscopy, x-ray diffraction, transmission electron microscopy, and scanning electron microscopy with energy-dispersive spectroscopy. The synthesized polydisperse ZnONPs were found to be in the size range of 8.9 to 32.6 nm, and at 18.0 µg ml exhibited maximum in vitro growth inhibition of the pathogen . . Scanning electron microscopy analysis of affected bacterial cells showed morphological deformation such as disruption of the cell membrane and wall, and the leakage of cell contents. Results of in vivo studies also showed that application of ZnONPs to the artificially inoculated tomato plants with the pathogen . significantly enhanced the plant growth by reducing bacterial soil population and disease severity as compared with the untreated control. Biosynthesized ZnONPs could be an effective approach to control the bacterium . .

摘要

花提取物被用作合成氧化锌纳米粒子(ZnONPs)的生物相容性材料。合成的 NPs 通过体外和体内实验评估其对革兰氏阴性菌的抗菌潜力,该菌会导致番茄和其他作物毁灭性的细菌性萎蔫病。通过紫外-可见光谱、傅里叶变换红外光谱、X 射线衍射、透射电子显微镜和扫描电子显微镜与能量色散光谱进一步分析合成的 ZnONPs。合成的多分散 ZnONPs 的尺寸范围为 8.9 至 32.6nm,在 18.0µg ml 时对病原体表现出最大的体外生长抑制。受影响细菌细胞的扫描电子显微镜分析显示出形态变形,如细胞膜和细胞壁的破坏以及细胞内容物的泄漏。体内研究的结果还表明,与未处理的对照相比,将 ZnONPs 应用于人工接种病原体的番茄植物上,显著增强了植物的生长,减少了土壤中的细菌数量和疾病严重程度。生物合成的 ZnONPs 可能是控制该细菌的有效方法。

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