miR-195 通过连接 lncRNA RUNX1-IT1 和 cyclin D1 来调节神经胶质瘤细胞的增殖。
MiR-195 connects lncRNA RUNX1-IT1 and cyclin D1 to regulate the proliferation of glioblastoma cells.
机构信息
Department of Neurosurgery, The Third People's Hospital of Datong City, Datong, P.R. China.
出版信息
Int J Neurosci. 2023 Jan;133(1):13-18. doi: 10.1080/00207454.2021.1881090. Epub 2021 Feb 3.
AIMS
LncRNA RUNX1-IT1 has been characterized as a tumor suppressive lncRNA in several cancers, while its role in glioblastoma (GBM) is unknown. This study aimed to investigate the potential involvement of RUNX1-IT1 in GBM.
METHODS
Expression of RUNX1-IT1 in GBM tissues and paired non-tumor tissues was determined by RT-qPCR. The interaction between RUNX1-IT1 and miR-195 was analyzed by dual luciferase activity assay. Overexpression of RUNX1-IT1 and miR-195 was achieved in GBM cells to explore the interaction between them. The effects of RUNX1-IT1 and miR-195 overexpression on the expression of cyclin D1 were analyzed by RT-qPCR and Western blot. Cell proliferation was analyzed by CCK-8 assay.
RESULTS
RUNX1-IT1 was upregulated in GBM. RUNX1-IT1 and miR-195 interacted with each other, but failed to regulate the expression of each other. Overexpression of RUNX1-IT1 resulted in the upregulation of cyclin D1, and also reduced the effects of miR-195 overexpression on cyclin D1 expression. RUNX1-IT1 and cyclin overexpression increased cell proliferation, while miR-195 overexpression decreased cell proliferation. In addition, RUNX1-IT1 overexpression reduced the effects of miR-195 overexpression on cell proliferation.
CONCLUSIONS
RUNX1-IT1 may sponge miR-195 to upregulate cyclin D1, thereby increasing the proliferation of glioblastoma cells.
目的
RUNX1-IT1 在几种癌症中被鉴定为一种肿瘤抑制性 lncRNA，但其在胶质母细胞瘤（GBM）中的作用尚不清楚。本研究旨在探讨 RUNX1-IT1 在 GBM 中的潜在作用。
方法
通过 RT-qPCR 测定 GBM 组织和配对非肿瘤组织中 RUNX1-IT1 的表达。通过双荧光素酶活性测定分析 RUNX1-IT1 与 miR-195 之间的相互作用。在 GBM 细胞中过表达 RUNX1-IT1 和 miR-195，以探讨它们之间的相互作用。通过 RT-qPCR 和 Western blot 分析 RUNX1-IT1 和 miR-195 过表达对 cyclin D1 表达的影响。通过 CCK-8 assay 分析细胞增殖。
结果
RUNX1-IT1 在 GBM 中上调。RUNX1-IT1 与 miR-195 相互作用，但未能调节彼此的表达。RUNX1-IT1 的过表达导致 cyclin D1 的上调，并且还降低了 miR-195 过表达对 cyclin D1 表达的影响。RUNX1-IT1 和 cyclin 的过表达增加了细胞增殖，而 miR-195 的过表达降低了细胞增殖。此外，RUNX1-IT1 的过表达降低了 miR-195 过表达对细胞增殖的影响。
结论
RUNX1-IT1 可能通过海绵吸附 miR-195 而上调 cyclin D1，从而增加胶质母细胞瘤细胞的增殖。
Zotero 插件