长链非编码 RNA DLGAP1-AS2 通过调控 miR-503/细胞周期蛋白 D1 促进非小细胞肺癌细胞增殖。
LncRNA DLGAP1-AS2 regulates miR-503/cyclin D1 to promote cell proliferation in non-small cell lung cancer.
机构信息
Department of Thoracic Surgery, Anhui Chest Hospital, No. 397 Jixi Road, Shushan District, Hefei City, Anhui Province, 230022, People's Republic of China.
出版信息
BMC Pulm Med. 2021 Aug 28;21(1):277. doi: 10.1186/s12890-021-01633-0.
BACKGROUND
LncRNA DLGAP1-AS2 plays an oncogenic role in glioma, while its role in other cancers is unknown. This study aimed to study the role of DLGAP1-AS2 in non-small cell lung cancer (NSCLC).
METHODS
Expression of DLGAP1-AS2 in NSCLC and paired non-tumor tissues from 64 NSCLC patients and the prognostic value of DLGAP1-AS2 for NSCLC were analyzed by performing a 5-year follow-up study. The interaction between DLGAP1-AS2 and miR-503 was confirmed by dual luciferase reporter assay, and their relationship was explored in NSCLC cells transfected with DLGAP1-AS2 expression vector or miR-503 mimic. The roles of DLGAP1-AS2 and miR-503 in regulating cyclin D1 expression were analyzed by RT-qPCR and Western blot. Cell proliferation was analyzed by CCK-8 assay.
RESULTS
DLGAP1-AS2 was upregulated in NSCLC and predicted poor survival. Interaction between DLGAP1-AS2 and miR-503 was confirmed by dual luciferase activity assay. Overexpression experiments showed that DLGAP1-AS2 and miR-503 overexpression failed to significantly affect the expression of each other. Interestingly, DLGAP1-AS2 overexpression upregulated cyclin D1, a target of miR-503, increased cell proliferation and reduced the effects of miR-503 overexpression on cyclin D1 expression and cell proliferation.
CONCLUSIONS
DLGAP1-AS2 may regulate miR-503/cyclin D1 to promote cell proliferation in NSCLC.
背景
长链非编码 RNA DLGAP1-AS2 在神经胶质瘤中发挥致癌作用，但其在其他癌症中的作用尚不清楚。本研究旨在研究 DLGAP1-AS2 在非小细胞肺癌（NSCLC）中的作用。
方法
通过对 64 例 NSCLC 患者的 NSCLC 及配对非肿瘤组织进行 5 年随访研究，分析 DLGAP1-AS2 在 NSCLC 中的表达及其对 NSCLC 的预后价值。通过双荧光素酶报告实验证实 DLGAP1-AS2 与 miR-503 的相互作用，并在转染 DLGAP1-AS2 表达载体或 miR-503 模拟物的 NSCLC 细胞中探讨它们的关系。通过 RT-qPCR 和 Western blot 分析 DLGAP1-AS2 和 miR-503 调节 cyclin D1 表达的作用。通过 CCK-8 测定分析细胞增殖。
结果
DLGAP1-AS2 在 NSCLC 中上调，预测生存不良。双荧光素酶活性测定证实了 DLGAP1-AS2 与 miR-503 之间的相互作用。过表达实验表明，DLGAP1-AS2 和 miR-503 的过表达均不能显著影响彼此的表达。有趣的是，DLGAP1-AS2 的过表达上调了 miR-503 的靶标 cyclin D1，增加了细胞增殖，并降低了 miR-503 过表达对 cyclin D1 表达和细胞增殖的影响。
结论
DLGAP1-AS2 可能通过调节 miR-503/cyclin D1 促进 NSCLC 中的细胞增殖。
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