Department of Thoracic Surgery, Anhui Chest Hospital, No. 397 Jixi Road, Shushan District, Hefei City, Anhui Province, 230022, People's Republic of China.
BMC Pulm Med. 2021 Aug 28;21(1):277. doi: 10.1186/s12890-021-01633-0.
LncRNA DLGAP1-AS2 plays an oncogenic role in glioma, while its role in other cancers is unknown. This study aimed to study the role of DLGAP1-AS2 in non-small cell lung cancer (NSCLC).
Expression of DLGAP1-AS2 in NSCLC and paired non-tumor tissues from 64 NSCLC patients and the prognostic value of DLGAP1-AS2 for NSCLC were analyzed by performing a 5-year follow-up study. The interaction between DLGAP1-AS2 and miR-503 was confirmed by dual luciferase reporter assay, and their relationship was explored in NSCLC cells transfected with DLGAP1-AS2 expression vector or miR-503 mimic. The roles of DLGAP1-AS2 and miR-503 in regulating cyclin D1 expression were analyzed by RT-qPCR and Western blot. Cell proliferation was analyzed by CCK-8 assay.
DLGAP1-AS2 was upregulated in NSCLC and predicted poor survival. Interaction between DLGAP1-AS2 and miR-503 was confirmed by dual luciferase activity assay. Overexpression experiments showed that DLGAP1-AS2 and miR-503 overexpression failed to significantly affect the expression of each other. Interestingly, DLGAP1-AS2 overexpression upregulated cyclin D1, a target of miR-503, increased cell proliferation and reduced the effects of miR-503 overexpression on cyclin D1 expression and cell proliferation.
DLGAP1-AS2 may regulate miR-503/cyclin D1 to promote cell proliferation in NSCLC.
长链非编码 RNA DLGAP1-AS2 在神经胶质瘤中发挥致癌作用，但其在其他癌症中的作用尚不清楚。本研究旨在研究 DLGAP1-AS2 在非小细胞肺癌（NSCLC）中的作用。
通过对 64 例 NSCLC 患者的 NSCLC 及配对非肿瘤组织进行 5 年随访研究，分析 DLGAP1-AS2 在 NSCLC 中的表达及其对 NSCLC 的预后价值。通过双荧光素酶报告实验证实 DLGAP1-AS2 与 miR-503 的相互作用，并在转染 DLGAP1-AS2 表达载体或 miR-503 模拟物的 NSCLC 细胞中探讨它们的关系。通过 RT-qPCR 和 Western blot 分析 DLGAP1-AS2 和 miR-503 调节 cyclin D1 表达的作用。通过 CCK-8 测定分析细胞增殖。
DLGAP1-AS2 在 NSCLC 中上调，预测生存不良。双荧光素酶活性测定证实了 DLGAP1-AS2 与 miR-503 之间的相互作用。过表达实验表明，DLGAP1-AS2 和 miR-503 的过表达均不能显著影响彼此的表达。有趣的是，DLGAP1-AS2 的过表达上调了 miR-503 的靶标 cyclin D1，增加了细胞增殖，并降低了 miR-503 过表达对 cyclin D1 表达和细胞增殖的影响。
DLGAP1-AS2 可能通过调节 miR-503/cyclin D1 促进 NSCLC 中的细胞增殖。
