长链非编码RNA RUNX1-IT1在子宫内膜癌中表达下调，并与miR-21前体结合以抑制其成熟。
LncRNA RUNX1-IT1 is Downregulated in Endometrial Cancer and Binds to miR-21 Precursor to Suppress Its Maturation.
作者信息
Liang Minglin, Wang Hongbo, Liu Cong, Lei Tao, Min Jie
机构信息
Department of Obstetrics and Gynecology, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan City, Hubei Province 430022, People's Republic of China.
出版信息
Cancer Manag Res. 2020 Dec 30;12:13451-13459. doi: 10.2147/CMAR.S272165. eCollection 2020.
BACKGROUND
RUNX1-IT1 suppresses colorectal cancer and liver cancer, while its role in other cancers is unknown. This study was performed to investigate the role of RUNX1-IT1 in endometrial cancer (EC).
METHODS
EC and paired non-tumor tissues were collected from 62 EC patients, and the expression of RUNX1-IT1, mature miR-21 and miR-21 precursor in these tissue samples were determined by RT-qPCR. Correlations were analyzed by linear regression. Overexpression of RUNX1-IT1 was achieved in EC cells and the expression of mature miR-21 and miR-21 precursor were analyzed by RT-qPCR. CCK-8 assay was used for cell proliferation analysis.
RESULTS
We found that RUNX1-IT1 was downregulated in EC and inversely correlated with mature miR-21 but not miR-21 precursor. RUNX1-IT1 was predicted to bind with miR-21 precursor. The interaction between them was verified by dual-luciferase activity assay and RNA pull-down assay. In EC cells, overexpression of RUNX1-IT1 downregulated mature miR-21, but not miR-21 precursor. Overexpression of RUNX1-IT1 suppressed the role of miR-21 in increasing cell proliferation.
CONCLUSION
RUNX1-IT1 is downregulated in EC and inhibits cancer cell proliferation by suppressing the maturation of miR-21.
背景
RUNX1-IT1可抑制结直肠癌和肝癌，但其在其他癌症中的作用尚不清楚。本研究旨在探讨RUNX1-IT1在子宫内膜癌（EC）中的作用。
方法
收集62例EC患者的EC组织及配对的非肿瘤组织，采用RT-qPCR检测这些组织样本中RUNX1-IT1、成熟miR-21和miR-21前体的表达。通过线性回归分析相关性。在EC细胞中实现RUNX1-IT1的过表达，并通过RT-qPCR分析成熟miR-21和miR-21前体的表达。采用CCK-8法进行细胞增殖分析。
结果
我们发现RUNX1-IT1在EC中表达下调，且与成熟miR-21呈负相关，但与miR-21前体无关。预测RUNX1-IT1与miR-21前体结合。通过双荧光素酶活性测定和RNA下拉测定验证了它们之间的相互作用。在EC细胞中，RUNX1-IT1的过表达下调了成熟miR-21，但未下调miR-21前体。RUNX1-IT1的过表达抑制了miR-21在增加细胞增殖中的作用。
结论
RUNX1-IT1在EC中表达下调，并通过抑制miR-21的成熟来抑制癌细胞增殖。
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