Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
Chem Commun (Camb). 2021 Feb 25;57(16):2041-2044. doi: 10.1039/d0cc07763a. Epub 2021 Jan 28.
We develop for the first time an aptamer-mediated rolling circle amplification approach for label-free and sensitive detection of histone-modifying enzyme (HME) activity. This method can achieve femtomolar sensitivity for histone acetyltransferase Tip60 assay, which is the most sensitive HME assay reported so far. It can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.
我们首次开发了一种适体介导的滚环扩增方法，用于无标记和敏感检测组蛋白修饰酶（HME）活性。该方法可实现对组蛋白乙酰转移酶 Tip60 检测的飞摩尔灵敏度，这是迄今为止报道的最灵敏的 HME 检测方法。它还可以进一步用于抑制剂筛选、酶动力学分析以及癌细胞内内源 Tip60 的测量。
