Wu Ruoyu, Guo Jiaping, Wang Minkai, Liu Huimin, Ding Lihua, Yang Ruiying, Liu Li-E, Liu Zhiyong
College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China.
Department of Neurosurgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, People's Republic of China.
ACS Omega. 2023 Apr 19;8(17):15741-15750. doi: 10.1021/acsomega.3c01408. eCollection 2023 May 2.
Ochratoxin A (OTA) is a common mycotoxin, and it is a significant threat to human health throughout the food chain. In this study, a sensitive and specific fluorescent sensor based on magnetic separation technology combined with chain displacement amplification was developed for fast and easy detection of OTA in food. The designed strand displacement amplification can improve the sensitivity for the detection, and the magnetic nanomaterials can provide a large surface area, thus enhancing the capture efficiency of the target from the sample. Based on those designs, the experimental results showed that the proposed method displayed excellent performance. The linearity range was 0.5-128.0 ng/mL. The detection limit was 0.125 ng/mL; the relative standard deviations were 3.92-7.71%. Additionally, the developed method was satisfactorily applied to determine OTA in wheat, corn, and red wine samples at three spiked levels (1.0, 8.0, and 64.0 ng/mL). The recoveries ranged from 85.45 to 107.8% for wheat flour, 101.34 to 108.35% for corn flour, and 91.15 to 93.80% for red wine, respectively. Compared with high-performance liquid chromatography, the proposed method showed a lower limit of detection and equal recovery. Hence, the designed method is a potential and good detecting tool for OTA residue analysis in complex matrix samples.
赭曲霉毒素A（OTA）是一种常见的霉菌毒素，对整个食物链中的人类健康构成重大威胁。在本研究中，开发了一种基于磁分离技术结合链置换扩增的灵敏且特异的荧光传感器，用于快速简便地检测食品中的OTA。所设计的链置换扩增可提高检测灵敏度，磁性纳米材料可提供大的表面积，从而提高从样品中捕获目标物的效率。基于这些设计，实验结果表明所提出的方法表现出优异的性能。线性范围为0.5 - 128.0 ng/mL。检测限为0.125 ng/mL；相对标准偏差为3.92 - 7.71%。此外，所开发的方法成功应用于测定小麦、玉米和红酒样品中三个加标水平（1.0、8.0和64.0 ng/mL）的OTA。小麦粉的回收率为85.45%至107.8%，玉米粉的回收率为101.34%至108.35%，红酒的回收率为91.15%至93.80%。与高效液相色谱法相比，所提出的方法检测限更低且回收率相当。因此，所设计的方法是用于复杂基质样品中OTA残留分析的一种潜在且良好的检测工具。
