Forrest Brasil Tecnologia Ltda, Araucaria, Brazil.
Faculty of Health Sciences of Barretos Dr Paulo Prata, Barretos, Brazil.
J Infect Dis. 2021 Sep 17;224(6):1005-1014. doi: 10.1093/infdis/jiab049.
There is a steady rise in the global incidence of Aedes-borne arbovirus disease. It has become urgent to develop alternative solutions for mosquito vector control. We developed a new method of sterilization of male mosquitoes with the goal to suppress a local Aedes aegypti population and to prevent the spread of dengue.
Sterile male mosquitoes were produced from a locally acquired Ae. aegypti colony by using a treatment that includes double-stranded RNA and thiotepa. A field study was conducted with sterile mosquito releases being performed on a weekly basis in predefined areas. There were 2 intervention periods (INT1 and INT2), with treatment and control areas reversed between INT1 and INT2.
During INT1, releases in the treated area resulted in up to 91.4% reduction of live progeny of field Ae. aegypti mosquitoes recorded over time, while the control neighborhoods (no releases of sterile male mosquitoes) remained highly infested. The successful implementations of the program during INT1 and INT2 were associated with 15.9-fold and 13.7-fold lower incidences of dengue in the treated area compared to the control areas, respectively.
Our data show the success of this new sterile insect technology-based program in preventing the spread of dengue.
全球登革热媒介蚊虫携带的虫媒病毒病发病率稳步上升。因此，迫切需要开发替代方案来控制蚊虫媒介。我们开发了一种新的雄性蚊虫绝育方法，旨在抑制局部埃及伊蚊种群并防止登革热传播。
采用双链 RNA 和噻替派联合处理，从当地获得的埃及伊蚊种群中产生不育雄蚊。在预先确定的区域内，每周进行一次不育雄蚊释放的现场研究。有 2 个干预期（INT1 和 INT2），在 INT1 和 INT2 之间反转处理区和对照区。
在 INT1 期间，处理区释放的不育雄蚊可使田间埃及伊蚊的活后代数量随时间减少高达 91.4%，而对照社区（未释放不育雄蚊）仍高度受感染。INT1 和 INT2 期间该计划的成功实施与处理区的登革热发病率比对照区分别低 15.9 倍和 13.7 倍相关。
我们的数据表明，这种基于新的不育昆虫技术的计划在防止登革热传播方面取得了成功。
