Departamento de Cirurgia E Fisiologia, Faculdade de Medicina da Universidade Do Porto, UnIC, Porto, Portugal.
Department of Medical Sciences, iBiMED, University of Aveiro, Aveiro, Portugal.
Cell Mol Life Sci. 2021 Apr;78(7):3285-3298. doi: 10.1007/s00018-020-03740-3. Epub 2021 Jan 28.
Micropeptides are small polypeptides coded by small open-reading frames. Progress in computational biology and the analyses of large-scale transcriptomes and proteomes have revealed that mammalian genomes produce a large number of transcripts encoding micropeptides. Many of these have been previously annotated as long noncoding RNAs. The role of micropeptides in cellular homeostasis maintenance has been demonstrated. This review discusses different types of micropeptides as well as methods to identify them, such as computational approaches, ribosome profiling, and mass spectrometry.
微肽是由小开放阅读框编码的小多肽。计算生物学的进展以及大规模转录组和蛋白质组的分析表明，哺乳动物基因组产生大量编码微肽的转录本。其中许多以前被注释为长非编码 RNA。微肽在细胞内稳态维持中的作用已得到证实。本文讨论了不同类型的微肽以及识别它们的方法，如计算方法、核糖体图谱和质谱。
