Endodontic Department São Leopoldo Mandic School of Dentistry, Rua José Rocha Junqueira 13, Campinas, SP, Brazil.
University of São Paulo, USP, São Paulo, Brazil.
Odontology. 2021 Jul;109(3):696-700. doi: 10.1007/s10266-021-00592-6. Epub 2021 Jan 28.
The aim of this study was to evaluate 3 different irrigation protocols using ozone on monoculture biofilm of Enterococcus faecalis. Fifty disto-buccal roots of maxillary first molars were instrumented up to the size 25.08, sterilized and then incubated with Enterococcus faecalis for 21 days for biofilm maturation in the root canal walls. After contamination, the initial collection (S1) was performed and the 50 samples were divided in 3 experimental groups (n = 15) and 1 positive control group (n = 5) as follows: Group1: Ozonated water (40 µg/ml); Group2: gaseous ozone (40 µg/ml); Group3: Ozonated water (40 µg/ml) under continuous ultrasonic irrigation (CUI) and, Group 4: 2.5% sodium hypochlorite (positive control). The final collection (S2) was performed using hedstroem files # 25 by scraping the root canal wall and absorbed paper cone. The collected samples were analyzed by CFU/ml count and qPCR. To compare the effect of irrigation methods and to investigate E. faecalis count data, Kruskal-Wallis and Wilcoxon nonparametric tests were used at p < 05. The values of the differences between the S1 values and the S2 values revealed that the groups did not differ significantly for either E. faecalis CFU count data (p = 0.713) or q-PCR (p = 0.185), however, it is observed that for all groups, the CFU count of E. faecalis decreased over 99%. For the q-PCR assay, the reduction was on the order of 74-95%. Only the NaOCl positive control group presented total elimination of CFU/mL bacterial counting. The ozone protocols assessed were similar in regard to Enterococcus faecalis reduction.
本研究旨在评估 3 种不同的臭氧冲洗方案对粪肠球菌单培养生物膜的作用。上颌第一磨牙的 50 个颊舌向远中根备至 25.08 号，经消毒后，在根管壁上孵育粪肠球菌 21 天，以成熟生物膜。污染后，进行初始采集（S1），将 50 个样本分为 3 个实验组（n=15）和 1 个阳性对照组（n=5），如下：组 1：臭氧水（40μg/ml）；组 2：气态臭氧（40μg/ml）；组 3：臭氧水（40μg/ml）联合连续超声冲洗（CUI），组 4：2.5%次氯酸钠（阳性对照）。使用 Hedstroem 扩孔锉#25 刮取根管壁和吸收性纸尖进行最终采集（S2）。用 CFU/ml 计数和 qPCR 分析收集的样本。为比较冲洗方法的效果并研究粪肠球菌计数数据，采用 Kruskal-Wallis 和 Wilcoxon 非参数检验，p<0.05。S1 值和 S2 值之间差异的效应值表明，各组粪肠球菌 CFU 计数数据（p=0.713）或 qPCR（p=0.185）均无显著差异，但所有组的粪肠球菌 CFU 计数均减少了 99%以上。qPCR 检测显示，减少量在 74%-95%之间。只有 NaOCl 阳性对照组的 CFU/mL 细菌计数完全消除。评估的臭氧方案在降低粪肠球菌方面相似。
