Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic., Campinas, SP, Brasil.
Graduate Program in Health Sciences, Pontifícia Universidade Católica de Campinas(PUC Campinas), Campinas, SP, Brasil.
Braz Dent J. 2023 May-Jun;34(3):33-41. doi: 10.1590/0103-6440202305318.
This in vitro study compared the antimicrobial efficacy of 2.5% sodium hypochlorite (NaOCl) and 8 µg/mL ozonated water agitated by passive ultrasonic irrigation (PUI) or PUI combined with EndoActivator (EA) against mature multispecies biofilm. One hundred and five oval-shaped mandibular premolars were instrumented, sterilized, and inoculated with Enterococcus faecalis, Candida albicans, and Staphylococcus aureus, divided into: control group - saline; O3 group - ozonated water; O3 PUI group - ozonated water with PUI agitation; O3 PUI+EA group - ozonated water with PUI+EA agitation; NaOCl group - NaOCl; NaOCl PUI group - NaOCl with PUI agitation; and NaOCl PUI+EA group - NaOCl with PUI+EA agitation. Microbiological samples were collected before (S1) and after (S2) the disinfection procedures and the data were statistically analyzed using the Kruskal-Wallis test. In the culture method, there was significant disinfection in the O3 PUI+EA, NaOCl, NaOCl PUI, and NaOCl PUI+EA groups (p˂0.05). The combination of NaOCl with PUI+EA reduced microbial counts to zero (p˂0.05). In the qPCR method, there was a significant reduction in the total count of viable microorganisms in the O3 PUI, O3 PUI+EA, NaOCl, NaOCl PUI, and NaOCl PUI+EA groups (p˂0.05). It can be concluded that 2.5% NaOCl with and without agitation, as well as 8 µg/mL ozonated water with its action enhanced by the agitation techniques, were effective in root canal disinfection, and their antimicrobial efficacy is related to the microorganisms present in the biofilm.
本体外研究比较了 2.5%次氯酸钠（NaOCl）和 8μg/mL 臭氧水在被动超声冲洗（PUI）或 PUI 联合 EndoActivator（EA）作用下对成熟多物种生物膜的抗菌效果。105 颗椭圆形下颌前磨牙被仪器化、消毒并接种粪肠球菌、白色念珠菌和金黄色葡萄球菌，分为：对照组-生理盐水；O3 组-臭氧水；O3 PUI 组-臭氧水+PUI 搅拌；O3 PUI+EA 组-臭氧水+PUI+EA 搅拌；NaOCl 组-NaOCl；NaOCl PUI 组-NaOCl+PUI 搅拌；和 NaOCl PUI+EA 组-NaOCl+PUI+EA 搅拌。在消毒程序前后（S1 和 S2）采集微生物样本，并使用 Kruskal-Wallis 检验对数据进行统计学分析。在培养方法中，O3 PUI+EA、NaOCl、NaOCl PUI 和 NaOCl PUI+EA 组的消毒效果显著（p˂0.05）。NaOCl 与 PUI+EA 联合使用可将微生物计数降至零（p˂0.05）。在 qPCR 方法中，O3 PUI、O3 PUI+EA、NaOCl、NaOCl PUI 和 NaOCl PUI+EA 组中总存活微生物计数显著减少（p˂0.05）。综上所述，2.5% NaOCl 无论是否搅拌，以及 8μg/mL 臭氧水经搅拌技术增强其作用，均可有效进行根管消毒，其抗菌效果与生物膜中存在的微生物有关。
