Department of Endodontics, Faculty of Dentistry, Süleyman Demirel University, Isparta, Turkey.
Lett Appl Microbiol. 2014 Jan;58(1):8-15. doi: 10.1111/lam.12148. Epub 2013 Oct 8.
This study aimed to compare the antimicrobial efficacy of low-temperature atmospheric pressure plasma (LTAPP) design and gaseous ozone delivery system with 2.5% NaOCl on Enterococcus faecalis in root canal walls and dentine tubules. The samples were divided into LTAPP (n = 12), ozone (n = 12), NaOCl (positive control, n = 12) and saline (negative control, n = 6) groups. Microbial samples were collected using paper points and dentin chips from root canals. Antimicrobial efficacy was assessed by counting the colony-forming units of Ent. faecalis before and after each irrigation protocol. Data were analysed using Kruskal-Wallis, Wilcoxon signed-rank, Friedman and Bonferroni t (Dunn's test)-tests (P = 0.05). The microbial sampling with paper points showed antibacterial efficacy of NaOCl, LTAPP, ozone and saline in descending order, respectively (P < 0.05). The microbial sampling with dentin chips demonstrated a superior efficacy of LTAPP compared with NaOCl in the middle third (P < 0.05), while both had similar effects in coronal and apical thirds (P > 0.05). NaOCl and LTAPP were better than ozone at the coronal and middle parts of the root canals (P < 0.05). These findings led us to suggest that LTAPP, which has no thermal and chemical effects, may be of great aid in endodontic treatment.
The present study handles different perspectives on chemomechanical preparation of root canals. Ozone and low-temperature atmospheric pressure plasma (LTAPP) were investigated to determine whether they could be an alternative for NaOCl. Up to now, chemical solutions (NaOCl, chlorhexidine digluconate, etc...) have been used to disinfect the root canals. When the reported effects of LTAPP on biological and chemical decontamination were taken into consideration, a question rose whether it has antimicrobial efficacy in root canals infected with E. faecalis. According to the findings of the present study, LTAPP may constitute a promising aid in endodontics in disinfection of root canals.
本研究旨在比较低温常压等离子体（LTAPP）设计和气体臭氧输送系统与 2.5%次氯酸钠对根管壁和牙本质小管内粪肠球菌的抗菌效果。样本分为 LTAPP（n = 12）、臭氧（n = 12）、次氯酸钠（阳性对照，n = 12）和盐水（阴性对照，n = 6）组。使用纸尖和牙本质屑从根管中采集微生物样本。使用平板计数法检测每个冲洗方案前后粪肠球菌的菌落形成单位，评估抗菌效果。数据采用 Kruskal-Wallis、Wilcoxon 符号秩、Friedman 和 Bonferroni t（Dunn 检验）检验（P = 0.05）。使用纸尖进行微生物采样显示，次氯酸钠、LTAPP、臭氧和盐水的抗菌效果依次降低（P < 0.05）。使用牙本质屑进行微生物采样显示，LTAPP 对根管中三分之一的效果优于次氯酸钠（P < 0.05），而在冠部和根尖三分之一的效果相似（P > 0.05）。次氯酸钠和 LTAPP 在根管的冠部和中部优于臭氧（P < 0.05）。这些发现表明，LTAPP 没有热和化学效应，可能对牙髓治疗有很大帮助。
本研究从不同角度处理根管的化学机械预备。研究了臭氧和低温常压等离子体（LTAPP），以确定它们是否可以替代次氯酸钠。到目前为止，化学溶液（次氯酸钠、葡萄糖酸氯己定等）已用于消毒根管。当考虑到 LTAPP 对生物和化学去污的报道效果时，就出现了一个问题，即在感染粪肠球菌的根管中，它是否具有抗菌效果。根据本研究的结果，LTAPP 可能在根管消毒方面构成牙髓治疗的一种有前途的辅助手段。
