State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, No. 7 Donghu South Road, Wuchang District, Wuhan, 430072, China.
University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, Beijing, 100049, China.
Microb Biotechnol. 2021 Mar;14(2):726-736. doi: 10.1111/1751-7915.13756. Epub 2021 Jan 28.
Sediment organic matter is a key stressor for submerged macrophyte growth, which negatively impacts the ecological restoration of lakes. Plant growth-promoting rhizobacteria (PGPR) were screened from the rhizosphere of submerged macrophytes and used due to their promoting effect on Vallisneria natans under a high sediment organic matter load. Root exudates were used as the sole carbon source to obtain the root affinity strains. Eight isolates were selected from the 61 isolated strains, based on the P solubilization, IAA production, cytokinins production and ACC deaminase activity. The analysis of the 16S rDNA indicated that one strain was Staphylococcus sp., while the other seven bacterial strains were Bacillus sp. They were all listed in low-risk groups for safety use in agricultural practices. The plant height significantly increased after inoculation with PGPR strains, with the highest rate of increase reaching 96%. This study provides an innovative technique for recovering submerged macrophytes under sediment organic matter stress.
沉积物有机质是影响沉水植物生长的关键胁迫因子，对湖泊的生态恢复有负面影响。从沉水植物根际筛选出植物促生根际细菌（PGPR），并在高沉积物有机质负荷下对苦草生长起到促进作用。利用根分泌物作为唯一碳源获得根亲和菌株。从 61 株分离株中选择了 8 株分离株，根据溶磷能力、IAA 产量、细胞分裂素产量和 ACC 脱氨酶活性进行筛选。16S rDNA 分析表明，一株为葡萄球菌属，其余 7 株为芽孢杆菌属。它们均被列入农业实践中安全使用的低风险组。接种 PGPR 菌株后，植物高度显著增加，最高增长率达到 96%。本研究为受沉积物有机质胁迫的沉水植物恢复提供了一种创新技术。
