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鉴定出一个编码类铁氧化还原蛋白的新日本慢生根瘤菌基因(frxA)。

Identification of a new Bradyrhizobium japonicum gene (frxA) encoding a ferredoxinlike protein.

作者信息

Ebeling S, Noti J D, Hennecke H

机构信息

Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zurich, Switzerland.

出版信息

J Bacteriol. 1988 Apr;170(4):1999-2001. doi: 10.1128/jb.170.4.1999-2001.1988.

Abstract

An open reading frame of 74 codons was identified downstream of the nifB gene of Bradyrhizobium japonicum 110. The predicted amino acid sequence shared 63% similarity with the Rhodopseudomonas palustris ferredoxin I sequence. We propose to name the gene frxA. The frxA gene was found to be cotranscribed with the nifB gene. An insertion mutation within frxA hardly affected nitrogen fixation activity.

摘要

在日本慢生根瘤菌110的nifB基因下游鉴定出一个由74个密码子组成的开放阅读框。预测的氨基酸序列与沼泽红假单胞菌铁氧化还原蛋白I序列有63%的相似性。我们提议将该基因命名为frxA。发现frxA基因与nifB基因共转录。frxA内的插入突变几乎不影响固氮活性。

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