追踪斑马鱼大脑中的单个细胞。

Tracking single cells in zebrafish brain.

机构信息

Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, China.

出版信息

J Neurosci Methods. 2021 Apr 1;353:109086. doi: 10.1016/j.jneumeth.2021.109086. Epub 2021 Jan 25.

DOI:10.1016/j.jneumeth.2021.109086

PMID:33508409

Abstract

Changes in cell locations and morphologies shape the brain. Tracking single cells over time is a vital step to study these changes, but densely arranged brain cells impede such observation. Larval zebrafish has become a popular model animal for single-cell tracking, owing to its small, transparent brain and easy genetic manipulation. In this article, we review recent single-cell tracking studies on neurons and non-neuronal cells in the larval zebrafish brain, including soma migration, process refinement, and interactions among cell types. These findings yield new insights regarding how the translocation and morphological changes of individual cells determine brain function.

摘要

细胞位置和形态的变化塑造了大脑。追踪细胞随时间的变化是研究这些变化的重要步骤，但密集排列的脑细胞阻碍了这种观察。幼鱼斑马鱼因其大脑小、透明且易于基因操作，已成为单细胞追踪的常用模式动物。本文综述了幼鱼斑马鱼大脑中神经元和非神经元细胞的单细胞追踪研究，包括胞体迁移、突起细化以及细胞类型间的相互作用。这些发现为个体细胞的移位和形态变化如何决定大脑功能提供了新的见解。