School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, Republic of Korea.
Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea.
Bioorg Med Chem Lett. 2021 Mar 15;36:127828. doi: 10.1016/j.bmcl.2021.127828. Epub 2021 Jan 26.
Bioactivity-driven LC/MS-based phytochemical analysis of the root bark extract of Ulmus davidiana var. japonica led to the isolation of 10 compounds including a new coumarin glycoside derivative, ulmusakidian (1). The structure of the new compound was elucidated using extensive spectroscopic analyses via 1D and 2D NMR spectroscopic data interpretations, HR-ESIMS, and chemical transformation. The isolated compounds 1-10 were tested for their antifungal activity against human fungal pathogens Cryptococcus neoformans and Candida albicans. Compounds 9 and 10 showed antifungal activity against C. neoformans, with the lowest minimal inhibitory concentration (MIC) of 12.5-25.0 µg/mL, whereas none of the compounds showed antifungal activity against C. albicans.
基于生物活性的 LC/MS 法对川鄂新榆根皮提取物的化学成分分析，分离得到了 10 种化合物，包括一个新的香豆素糖苷衍生物，川鄂新榆苷（1）。通过 1D 和 2D NMR 光谱数据分析、高分辨电喷雾质谱（HR-ESIMS）和化学转化等手段，对新化合物的结构进行了详细解析。对分离得到的化合物 1-10 进行了抗人类真菌病原体新型隐球菌和白色念珠菌的抗真菌活性测试。化合物 9 和 10 对新型隐球菌表现出抗真菌活性，最低抑菌浓度（MIC）为 12.5-25.0µg/mL，而对白色念珠菌没有表现出抗真菌活性。
