三（2-氯乙基）磷酸酯（TCEP）在环境相关浓度下对斑马鱼（Danio rerio）早期生活阶段的影响。
Effects of environmentally relevant concentrations of tris (2-chloroethyl) phosphate (TCEP) on early life stages of zebrafish (Danio rerio).
机构信息
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China.
出版信息
Environ Toxicol Pharmacol. 2021 Apr;83:103600. doi: 10.1016/j.etap.2021.103600. Epub 2021 Jan 26.
Tris (2-chloroethyl) phosphate (TCEP) has been received great concerns because of its increasing presence in various environmental compartments and toxicity. In the present study, zebrafish embryos were exposed to environmentally relevant concentrations of TCEP (0.2, 2, 20, 200 μg/L) from 3 to 120 h post-fertilization (hpf). The results showed that TCEP exposure (20, 200 μg/L) led to developmental toxicity including decreased body length and delay of hatching. Treatment with TCEP significantly decreased whole-body thyroxine (T4) levels and mRNA level of thyroglobulin (tg), and enhanced transcriptions of genes sodium/iodide symporter (nis), thyroid hormone receptor α (trα) and ugt1ab involved in thyroid synthesis and metabolism, respectively. Additionally, TCEP altered the transcription of α1-tubulin, gap43 and mbp related to nervous system development, even at relatively low concentrations. Overall, our results revealed that TCEP exposure can lead to developmental toxicity, thyroid endocrine disruption and neurotoxicity on early developmental stages of zebrafish.
三（2-氯乙基）磷酸酯（TCEP）因其在各种环境介质中的广泛存在和毒性而受到广泛关注。本研究采用斑马鱼胚胎为实验对象，从受精后 3 小时至 120 小时（hpf），暴露于环境相关浓度的 TCEP（0.2、2、20、200μg/L）中。结果表明，TCEP 暴露（20、200μg/L）导致胚胎发育毒性，包括体长减小和孵化延迟。TCEP 处理显著降低了鱼体的总甲状腺素（T4）水平和甲状腺球蛋白（tg）的 mRNA 水平，并分别增强了甲状腺合成和代谢相关基因钠/碘转运体（nis）、甲状腺激素受体α（trα）和 ugt1ab 的转录。此外，TCEP 还改变了与神经系统发育相关的α1-微管蛋白、gap43 和 mbp 的转录，即使在较低的浓度下也是如此。综上所述，本研究结果表明，TCEP 暴露可导致斑马鱼早期发育阶段的发育毒性、甲状腺内分泌干扰和神经毒性。
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