Exposure to tris (1,3-dichloro-2-propyl) phosphate affects the embryonic cardiac development of .

Tris (1,3-dichloro-2-propyl) phosphate (TDCPP) is a growing concern and may be a potential risk to marine environmental health due to its widespread usage and distribution. However, the toxic effects of TDCPP on cardiac development in marine fish have not been reported. In this study, embryos were exposed to TDCPP at doses of 0, 0.04, 0.4, 4 and 40 μg/L from early embryogenesis to 10 days postfertilization (dpf). Then, the heart rate and sinus venosus-bulbus arteriosus (SV-BA) distance of the exposed embryos were measured at 5, 6, 8 and 10 dpf. Furthermore, alterations in the mRNA levels of the genes encoding cyclooxygenase-2 (), bone morphogenetic protein 4 (), fibroblast growth factor 8 (), and GATA-binding protein 4 () were evaluated at 5, 6, 8 and 10 dpf. We found that the heart rate significantly increased in all TDCPP exposure groups at 10 dpf. The SV-BA distance significantly decreased in all TDCPP exposure groups at all developmental stages (except for the 0.4 μg/L group at 5 dpf and the 4 μg/L group at 10 dpf). The mRNA expression of was downregulated at 5 dpf was downregulated at 5 and 6 dpf, was downregulated at 5, 6 and 8 dpf, was downregulated at 8 dpf, and was upregulated at 10 dpf. These results indicate that the changes in heart rate and SV-BA distance might be accompanied by disturbances in the four genes involved in cardiac development. Our findings will help to illustrate the possible cardiac toxic effects of marine fish exposed to TDCPP.