Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, 130012, PR China.
China-Japan Union Hospital of Jilin University, Changchun, 130033, PR China.
Colloids Surf B Biointerfaces. 2021 Apr;200:111584. doi: 10.1016/j.colsurfb.2021.111584. Epub 2021 Jan 20.
Nanopeptide assembled from peptide-anchored nanoparticles possess an enormous research potential in the field of cellular medicine and disease treatment. The aim of this study was to explore the neuroprotective effects of maize tetrapeptide anchored gold nanoparticles against l-glutamic acid-induced PC12 cell apoptosis and a murine Alzheimer's disease model induced by aluminum chloride and d-galactose. The results revealed that the nanopeptide antioxidant inhibited intracellular ROS accumulation and promoted cell differentiation than that of maize bioactive tetrapeptide. Compared with untreated Alzheimer's disease model mice, nanopeptide administration shortened the escape latency time in a water maze test and improved the movements in the autonomic activity test. After 16 days of nanopeptide administration, the central cholinergic system function of acetylcholine and cholineacetyltransferase were enhanced, and the level of acetylcholinesterase was reduced. It also increased superoxide dismutase and glutathione peroxidase activity in sera and hypothalami. Moreover, nanopeptide treatment upregulated cerebral nuclear factor erythroid 2-related factor 2 and heme-oxygenase-1 and downregulated kelch-like ECH-associated protein 1 relative to untreated Alzheimer's disease model mice. Thus, the novel nanopeptide is expected to be used as the neuroprotective agent to prevent Alzheimer's disease.
由肽锚定纳米颗粒组装而成的纳米肽在细胞医学和疾病治疗领域具有巨大的研究潜力。本研究旨在探讨玉米四肽锚定金纳米颗粒对 l-谷氨酸诱导的 PC12 细胞凋亡和氯化铝和半乳糖诱导的小鼠阿尔茨海默病模型的神经保护作用。结果表明，纳米肽抗氧化剂抑制了细胞内 ROS 的积累，并促进了细胞分化，优于玉米生物活性四肽。与未治疗的阿尔茨海默病模型小鼠相比，纳米肽给药缩短了水迷宫测试中的逃避潜伏期时间，并改善了自主活动测试中的运动。纳米肽给药 16 天后，增强了乙酰胆碱和胆碱乙酰转移酶的中枢胆碱能系统功能，降低了乙酰胆碱酯酶的水平。它还增加了血清和下丘脑中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。此外，与未治疗的阿尔茨海默病模型小鼠相比，纳米肽治疗上调了脑核因子红细胞 2 相关因子 2 和血红素加氧酶-1，并下调了 Kelch 样 ECH 相关蛋白 1。因此，新型纳米肽有望作为神经保护剂用于预防阿尔茨海默病。
