贻贝（Mytilus galloprovincialis）暴露于镉中时 microRNAs 表达中的应激反应。
Stress responses in expressions of microRNAs in mussel Mytilus galloprovincialis exposed to cadmium.
机构信息
Center for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, PR China; Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, PR China.
Center for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR China.
出版信息
Ecotoxicol Environ Saf. 2021 Apr 1;212:111927. doi: 10.1016/j.ecoenv.2021.111927. Epub 2021 Jan 25.
MicroRNAs (miRNAs) are known to have complicated functions in aquatic species, but little is known about the role of miRNAs in mollusk species under environmental stress. In this study, we performed small RNA sequencing to characterize the differentially expressed miRNAs in different tissues (whole tissues, digestive glands, gills, and gonads) of blue mussels (Mytilus galloprovincialis) exposed to cadmium (Cd). In summary, 107 known miRNAs and 32 novel miRNAs were significantly (p < 0.01) differentially expressed after Cd exposure. The peak size of miRNAs was 22 nucleotides. Target genes of these differentially expressions of miRNAs related to immune defense, apoptosis, lipid and xenobiotic metabolism showed significant changes under Cd stress. These findings provide the first characterization of miRNAs in mussel M. galloprovincialis and expressions of many target genes in response to Cd stress.
微 RNA（miRNAs）在水生物种中具有复杂的功能，但对贝类物种在环境胁迫下的 miRNA 作用知之甚少。在这项研究中，我们进行了小 RNA 测序，以表征暴露于镉（Cd）的贻贝（Mytilus galloprovincialis）不同组织（整体组织、消化腺、鳃和性腺）中差异表达的 miRNAs。总的来说，107 个已知 miRNAs 和 32 个新的 miRNAs 在 Cd 暴露后显著（p < 0.01）差异表达。miRNAs 的峰大小为 22 个核苷酸。这些差异表达的 miRNAs 的靶基因与免疫防御、细胞凋亡、脂质和外来化合物代谢相关，在 Cd 胁迫下显示出显著变化。这些发现首次对贻贝 M. galloprovincialis 的 miRNAs 进行了特征描述，并对许多靶基因对 Cd 胁迫的反应进行了描述。
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