Huang Yan, Xu Tailin, Luo Yong, Liu Conghui, Gao Xuan, Cheng Zhihao, Wen Yongqiang, Zhang Xueji
Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
School of Biomedical Engineering, Shenzhen University, Shenzhen, Guangdong 518060, P. R. China.
Anal Chem. 2021 Feb 9;93(5):2996-3001. doi: 10.1021/acs.analchem.0c05032. Epub 2021 Jan 29.
Lateral flow biosensor (LFB) is one of the most successful and applied commercial detection methods for food safety, drug abuse, and disease. Here, we integrated the ultrasound enrichment as sample preparation with LFB to achieve the ultra-trace protein detection in blood. When the ultrasound field is applied, the interaction between the acoustic field and gold nanoparticles can gather specifically modified gold nanoparticles toward pressure nodes in seconds and enrich target proteins. Such an approach can detect protein with a linear range of 1-20 ng mL and detection limit of 0.58 ng mL in blood within 20 min, which enormously reduces false positive readings caused by interference in real blood samples with complex components. Such a microchip that integrated acoustic enrichment with LFB shows great potential in detecting ultra-trace biomarkers for clinical diagnosis.
侧向流动生物传感器（LFB）是食品安全、药物滥用和疾病检测领域最成功且应用广泛的商业检测方法之一。在此，我们将超声富集作为样品制备方法与LFB相结合，以实现血液中痕量蛋白质的检测。当施加超声场时，声场与金纳米颗粒之间的相互作用可在数秒内将特异性修饰的金纳米颗粒聚集到压力节点处，从而富集目标蛋白质。这种方法能够在20分钟内检测出血液中线性范围为1 - 20 ng/mL且检测限为0.58 ng/mL的蛋白质，极大地减少了由复杂成分的实际血液样本中的干扰导致的假阳性读数。这种将声学富集与LFB集成的微芯片在检测用于临床诊断的超痕量生物标志物方面显示出巨大潜力。
