Siavashifar Marzieh, Rezaei Fatemeh, Motallebirad Tahereh, Azadi Davood, Absalan Abdorrahim, Naserramezani Zahra, Golshani Mohadeseh, Jafarinia Morteza, Ghaffari Kazem
Student Research Comitee, Khomein University of Medical Sciences, Khomein, Iran.
Department of Basic and Laboratory and Sciences, Khomein University of Medical Sciences, Qods street, Khomein, Iran.
Genes Environ. 2021 Jan 28;43(1):2. doi: 10.1186/s41021-021-00173-7.
Hospital environmental resources have a significant role in cross-transmission of opportunistic pathogens such as actinomycetes species to the patients. Actinomycetes have a remarkable capability to survive in adverse and harsh conditions of hospital environments; therefore, they are a threat to the health of patients. Due to this issue, we aimed to determine the frequency and diversity of actinomycetes species in hospital soil, water and dust by using a combination of conventional and molecular methods including the phenotypic and biochemical tests for preliminary identification and the PCR amplification of the specific region of the 16S rRNA, hsp65 gene and sequence analyses of 16S rRNA for the genus and species identification.
A total of 50 (35.2%) actinomycetes isolates from 7 genera were isolated from 142 hospital environmental samples. The three most prevalent species were M. setense 10%, R. erythropolis and M. fortuitum 8% followed by N.cyriacigeorgica and M. gordonae 6%, M. chelonae, M. abscessus, M. lentiflavum, M. mucogenicum, N. asteroides, N. farcinica, R. equi and L. shinushuensis 4% and the single isolates of M. conceptionense, M. septicum, N. rhamnosophilia, N. bravicatena, M. flavescens, M. arupense, M. doricum, M. frederiksbergense, S. heliomycini, S. albus, S. albogriseolus, R. facians, D. maris, G. terae and A. globiformis.
In conclusion we showed that the hospital environment is a potential reservoir for a broad range of actinomycetes species, due to the remarkable survival capability of these microorganisms in adverse hospital environment, carrying a threat to the health of patients.
医院环境资源在诸如放线菌等机会性病原体向患者的交叉传播中起着重要作用。放线菌具有在医院环境的不利和恶劣条件下生存的显著能力；因此，它们对患者健康构成威胁。鉴于此问题，我们旨在通过结合传统方法和分子方法来确定医院土壤、水和灰尘中放线菌的频率和多样性，这些方法包括用于初步鉴定的表型和生化测试，以及对16S rRNA特定区域、hsp65基因的PCR扩增和用于属及种鉴定的16S rRNA序列分析。
从142份医院环境样本中分离出了来自7个属的共50株（35.2%）放线菌。三种最常见的菌种是赛滕分枝杆菌（10%）、红平红球菌和偶然分枝杆菌（8%），其次是乔治亚新诺卡氏菌和戈登分枝杆菌（6%）、龟分枝杆菌、脓肿分枝杆菌、淡黄分枝杆菌、产黏液分枝杆菌、星状诺卡氏菌、豚鼠耳炎诺卡氏菌、马红球菌和新竹链霉菌（4%），以及概念分枝杆菌、败血分枝杆菌、鼠诺卡氏菌、勇敢链霉菌、微黄分枝杆菌、阿鲁普分枝杆菌、多里卡分枝杆菌、腓特烈斯贝格分枝杆菌、日光链霉菌、白色链霉菌、白灰链霉菌、颜面玫瑰色杆菌、海氏动性球菌、土壤戈登氏菌和球形放线菌的单株分离菌。
总之，我们表明医院环境是多种放线菌的潜在储存库，由于这些微生物在不利的医院环境中具有显著的生存能力，对患者健康构成威胁。
