van der Ven H H, Jeyendran R S, Al-Hasani S, Tünnerhoff A, Hoebbel K, Diedrich K, Krebs D, Perez-Pelaez M
Department of Obstetrics and Gynaecology, University of Bonn, FRG.
Hum Reprod. 1988 Jan;3(1):85-8. doi: 10.1093/oxfordjournals.humrep.a136659.
The number and viability of spermatozoa recovered by glass wool column filtration and a swim-up procedure were compared using different types of ejaculates, such as normal, asthenozoospermic and very viscous oligozoospermic semen. The filtration procedure resulted in significantly (P less than 0.01) higher recovery of viable spermatozoa than the swim-up procedure from all types of ejaculates studied. Further, the spermatozoa from 50 (78.1%) of the 64 ejaculates filtered through glass wool column fertilized at least one intact human egg in an in-vitro fertilization (IVF) procedure. It is concluded that glass wool column filtration is superior to the swim-up procedure since it yields a higher recovery of viable spermatozoa that are potentially fertile. Therefore, the glass wool column filtration procedure used to prepare spermatozoa may be of benefit for IVF, intra-uterine insemination, in-vitro fertilization and GIFT (gamete intra-Fallopian transfer), especially in cases of poor quality semen.
使用玻璃棉柱过滤和上游法，对通过不同类型射精（如正常、弱精子症和高粘度少精子症精液）回收的精子数量和活力进行了比较。在所有研究的射精类型中，过滤程序回收的活精子数量显著高于上游法（P<0.01）。此外，在体外受精（IVF）程序中，通过玻璃棉柱过滤的64份射精中的50份（78.1%）的精子使至少一个完整的人类卵子受精。结论是，玻璃棉柱过滤优于上游法，因为它能回收更多具有潜在生育能力的活精子。因此，用于制备精子的玻璃棉柱过滤程序可能对IVF、宫内授精、体外受精和配子输卵管内移植（GIFT）有益，尤其是在精液质量差的情况下。
