al-Hasani S, Alpüstün S, Ludwig M, Diedrich K, Bauer O, Küpker W, Wolff A, Krebs D
Department of Obstetrics and Gynecology, Medical University Lübeck, Germany.
Int J Androl. 1996 Feb;19(1):55-60. doi: 10.1111/j.1365-2605.1996.tb00435.x.
The aim of this prospective study was to relate sperm quality, especially sperm morphology, to the outcome of IVF results, when glass wool filtration (GWF) and swim-up were used in combination for the preparation of spermatozoa. A total of 60 ejaculates were analysed. GWF was compared with glass wool filtration/swim-up (GWF-SU) by using aliquots from the same semen samples to increase the precision of the comparison and to establish the cumulative effect of these two semen preparation methods on the morphology of the recovered spermatozoa. Sperm parameters were examined in native semen, in semen preparation samples after GWF and GWF-SU. The mean percentages for motility, morphology and velocity were improved significantly over those in fresh semen only when GWF-SU was used. GWF alone resulted only in a significantly higher recovery of motile spermatozoa. In semen preparation, 10% of spermatozoa with normal morphology appeared to be the cut-off point as there was a significantly higher recovery in fresh semen samples containing > 10% morphologically normal spermatozoa after GWF-SU. Outcome of IVF-ET following preparation with GWF-SU showed better results in comparison to the swim-up procedure alone, though this difference was not statistically significant. The cut-off point was also > 10% morphologically normal spermatozoa. Improvement in all aspects of IVF-ET occurred when native semen contained > 10% morphologically normal spermatozoa. It is concluded that GWF alone did not produce significantly better results but, in combination with swim-up, it resulted in significantly better sperm morphology and in an improved outcome of IVF-ET for fresh semen samples with > 10% morphologically normal spermatozoa.
这项前瞻性研究的目的是，在使用玻璃棉过滤（GWF）和上游法联合制备精子时，将精子质量，尤其是精子形态，与体外受精（IVF）结果相关联。共分析了60份射精样本。通过使用来自相同精液样本的等分试样，将GWF与玻璃棉过滤/上游法（GWF-SU）进行比较，以提高比较的精确度，并确定这两种精液制备方法对回收精子形态的累积影响。在未处理精液、GWF处理后的精液制备样本以及GWF-SU处理后的精液制备样本中检测精子参数。仅在使用GWF-SU时，活力、形态和速度的平均百分比相较于新鲜精液有显著改善。单独使用GWF仅导致活动精子的回收率显著更高。在精液制备中，形态正常的精子占10%似乎是一个临界点，因为在GWF-SU处理后，形态正常精子含量>10%的新鲜精液样本中，精子回收率显著更高。与单独使用上游法相比，GWF-SU处理后进行体外受精-胚胎移植（IVF-ET）的结果显示出更好的效果，尽管这种差异在统计学上并不显著。临界点同样是形态正常的精子>10%。当未处理精液中形态正常的精子>10%时，IVF-ET的各个方面都有所改善。得出的结论是，单独使用GWF不会产生显著更好的结果，但与上游法联合使用时，对于形态正常精子>10%的新鲜精液样本，可显著改善精子形态，并提高IVF-ET的结果。
