Wang Shengnan, Ge Fei, Cai Tianyu, Qi Shimei, Qi Zhilin
Department of Biochemistry and Molecular Biology, Wannan Medical College, Wuhu 241002, China.
Anhui Provincial Key Laboratory of Active Biological Macro-molecules, Wannan Medical College, Wuhu 241002, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2021 Jan 30;41(1):87-92. doi: 10.12122/j.issn.1673-4254.2021.01.12.
To investigate the inhibitory effects of dihydromyricetin on the proliferation and migration of gastric cancer BGC-823 cells and explore the molecular mechanisms.
BGC-823 cells in routine culture were treated with different concentrations of dihydromyricetin (0, 40, 60, 80, 100, and 120 μg/mL) for 24 h, and the changes in cell viability were detected using CCK-8 assay; colony forming assay and Transwell assay were performed to assess the changes in colonyforming and migration abilities of the cells, respectively. The levels of MMP-2 and MMP-9 in the treated cells were determined using ELISA, and Western blotting was used to detect the expressions of E-cadherin, N-cadherin, cyclin D1, cyclin E1, HSP70 and HMGB1 and the phosphorylation levels of Akt and Stat3.
CCK-8 assay showed that dihydromyricetin treatment dose-dependently inhibited the viability of BGC-823 cells ( < 0.05). Treatment with dihydromyricetin obviously suppressed the proliferation and migration of BGC-823 cells, significantly reduced the expression levels of cyclin D1, cyclin E1 and Ncadherin, enhanced E-cadherin expression, inhibited the phosphorylation of Akt and stat3, and downregulated HMGB1 expression in the cells. The results of ELISA demonstrated significantly lowered levels of MMP-2 and MMP-9 in dihydromyricetin-treated cells.
Dihydromyricetin inhibits the proliferation and migration of BGC-823 cells through suppressing the activation of Akt/stat3 signaling pathways and HMGB1 expression.
研究二氢杨梅素对胃癌BGC - 823细胞增殖和迁移的抑制作用，并探讨其分子机制。
将常规培养的BGC - 823细胞用不同浓度的二氢杨梅素（0、40、60、80、100和120μg/mL）处理24小时，采用CCK - 8法检测细胞活力变化；分别进行集落形成试验和Transwell试验评估细胞集落形成和迁移能力的变化。用ELISA法测定处理后细胞中MMP - 2和MMP - 9的水平，用蛋白质印迹法检测E - 钙黏蛋白、N - 钙黏蛋白、细胞周期蛋白D1、细胞周期蛋白E1、热休克蛋白70（HSP70）和高迁移率族蛋白B1（HMGB1）的表达以及Akt和Stat3的磷酸化水平。
CCK - 8法显示，二氢杨梅素处理呈剂量依赖性抑制BGC - 823细胞活力（<0.05）。二氢杨梅素处理明显抑制BGC - 823细胞的增殖和迁移，显著降低细胞周期蛋白D1、细胞周期蛋白E1和N - 钙黏蛋白的表达水平，增强E - 钙黏蛋白表达，抑制Akt和Stat3的磷酸化，并下调细胞中HMGB1的表达。ELISA结果表明，二氢杨梅素处理的细胞中MMP - 2和MMP - 9水平显著降低。
二氢杨梅素通过抑制Akt/Stat3信号通路的激活和HMGB1表达来抑制BGC - 823细胞的增殖和迁移。
