Gutkind J S, Kurihara M, Saavedra J M
Unit on Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, Maryland 20892.
Am J Physiol. 1988 Sep;255(3 Pt 2):H646-50. doi: 10.1152/ajpheart.1988.255.3.H646.
We analyzed angiotensin II (ANG II) receptors by in vitro autoradiography in selective brain nuclei of control, salt-treated (1% NaCl in drinking water), deoxycorticosterone acetate (DOCA)-treated (DOCA pivalate, 25 mg/kg sc weekly), and DOCA-salt-treated (DOCA + salt treatments) uninephrectomized male Wistar-Kyoto rats. After 4 wk of treatment, only the DOCA-salt group developed hypertension. ANG II binding increased in median preoptic nucleus and subfornical organ of salt- and DOCA-treated rats. DOCA-treated rats also showed increased ANG II binding in paraventricular nucleus. DOCA-salt-treated rats showed higher ANG II binding in nucleus of the solitary tract and area postrema, as well as in the areas mentioned before. Although salt and/or DOCA treatments alone increased ANG II receptors in some brain nuclei, after combined DOCA-salt treatment there was significantly higher ANG II binding in all areas, except the median preoptic nucleus. These results suggest that increased ANG II receptors in selected brain areas may play a role in the pathophysiology of mineralocorticoid-salt experimental hypertension.
我们通过体外放射自显影术，分析了未切除单侧肾脏的雄性Wistar-Kyoto大鼠在对照、盐处理（饮用水中含1%氯化钠）、醋酸脱氧皮质酮（DOCA）处理（新戊酸脱氧皮质酮，每周皮下注射25 mg/kg）以及DOCA-盐处理（DOCA + 盐处理）后的选择性脑核中的血管紧张素II（ANG II）受体。治疗4周后，只有DOCA-盐组出现了高血压。在盐处理和DOCA处理的大鼠中，视前正中核和穹窿下器官的ANG II结合增加。DOCA处理的大鼠在室旁核中也显示出ANG II结合增加。DOCA-盐处理的大鼠在孤束核和最后区以及之前提到的区域中显示出更高的ANG II结合。尽管单独的盐和/或DOCA处理会增加某些脑核中的ANG II受体，但在DOCA-盐联合处理后，除视前正中核外，所有区域的ANG II结合均显著更高。这些结果表明，特定脑区中ANG II受体的增加可能在盐皮质激素-盐实验性高血压的病理生理学中起作用。
