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比较野生和家养中华树鼩（Tupaia belangeri chinensis）海马体 microRNA 表达谱。

Comparing the hippocampal miRNA expression profiles of wild and domesticated Chinese tree shrews (Tupaia belangeri chinensis).

机构信息

Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, China.

Yunnan Key Laboratory of Vaccine Research and Development On Severe Infectious Diseases, Kunming, China.

出版信息

BMC Ecol Evol. 2021 Jan 25;21(1):12. doi: 10.1186/s12862-020-01740-2.

DOI:10.1186/s12862-020-01740-2
PMID:33514308
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7853310/
Abstract

BACKGROUND

The domestication of tree shrews represents an important advance in the development of standardized laboratory animals. Little is known regarding the miRNA changes that accompany the transformation of wild tree shrews into domestic tree shrews.

RESULTS

By performing miRNA-seq analysis on wild and domestic tree shrews, we identified 2410 miRNAs and 30 differentially expressed miRNAs in the hippocampus during tree shrew domestication. A KEGG analysis of the differentially expressed genes showed that the differentially expressed miRNAs were associated with ECM-receptor interaction, the phosphatidylinositol signaling system, protein digestion and absorption, inositol phosphate metabolism, lysine degradation, fatty acid degradation and focal adhesion. Most of these pathways could be classified under environmental information processing, organismal systems and metabolism. The miRNAs exclusively expressed in wild and tame tree shrews GO enriched in terms of divergent functions. The miRNA-mRNA networks suggested that novel-m1388-5p and novel-m0746-5p might play regulatory roles in domestication of tree shrews. Real-time RT-PCR analysis was employed to verify the presence of these miRNAs.

CONCLUSION

We identified a number of candidate miRNA-regulated domestication genes that may represent targets for selection during the domestication of tree shrews.

摘要

背景

树鼩的驯化是标准化实验动物发展的重要进步。对于伴随野生树鼩向家养树鼩转变而发生的 miRNA 变化，人们知之甚少。

结果

通过对野生和家养树鼩的 miRNA-seq 分析，我们在树鼩驯化过程中鉴定出了 2410 个 miRNA 和 30 个差异表达的 miRNA 在海马体中。差异表达基因的 KEGG 分析表明，差异表达的 miRNA 与 ECM-受体相互作用、磷脂酰肌醇信号系统、蛋白质消化吸收、肌醇磷酸盐代谢、赖氨酸降解、脂肪酸降解和黏附有关。这些途径大部分可分为环境信息处理、机体系统和代谢。仅在野生和驯化树鼩中表达的 miRNA 的 GO 富集术语在功能上存在差异。miRNA-mRNA 网络表明，novel-m1388-5p 和 novel-m0746-5p 可能在树鼩驯化中发挥调节作用。实时 RT-PCR 分析验证了这些 miRNA 的存在。

结论

我们鉴定出了一些候选 miRNA 调控的驯化基因，这些基因可能代表了树鼩驯化过程中的选择靶标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/c15ff4b6b9b2/12862_2020_1740_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/8db7409ed1f1/12862_2020_1740_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/231adffa77e4/12862_2020_1740_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/6367e2e275c5/12862_2020_1740_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/b63a268dc889/12862_2020_1740_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/6244193d8b3f/12862_2020_1740_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/c15ff4b6b9b2/12862_2020_1740_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/8db7409ed1f1/12862_2020_1740_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/231adffa77e4/12862_2020_1740_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/6367e2e275c5/12862_2020_1740_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/b63a268dc889/12862_2020_1740_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/6244193d8b3f/12862_2020_1740_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9927/7853310/c15ff4b6b9b2/12862_2020_1740_Fig6_HTML.jpg

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