Mouse Epigenetics Project, ISS/Kibo Experiment, Japan Aerospace Exploration Agency, Tsukuba, Japan.
JEM Utilization Center, Human Spaceflight Technology Directorate, Japan Aerospace Exploration Agency, Tsukuba, Ibaraki, Japan.
Sci Rep. 2021 Jan 29;11(1):2665. doi: 10.1038/s41598-021-82013-w.
Many experiments have analyzed the effect of the space environment on various organisms. However, except for the group-rearing of mice in space, there has been little information on the behavior of organisms in response to gravity changes. In this study, we developed a simple Active Inactive Separation (AIS) method to extract activity and inactivity in videos obtained from the habitat cage unit of a space experiment. This method yields an activity ratio as a ratio of 'activity' within the whole. Adaptation to different gravitational conditions from 1g to hypergravity (HG) and from microgravity (MG) to artificial 1g (AG) was analyzed based on the amount of activity to calculate the activity ratio and the active interval. The result for the activity ratios for the ground control experiment using AIS were close to previous studies, so the effectiveness of this method was indicated. In the case of changes in gravity from 1g to HG, the ratio was low at the start of centrifugation, recovered sharply in the first week, and entered a stable period in another week. The trend in the AG and HG was the same; adapting to different gravity environments takes time.
许多实验分析了空间环境对各种生物的影响。然而，除了在太空中进行的老鼠群体饲养实验，很少有关于生物对重力变化反应的行为信息。在本研究中，我们开发了一种简单的主动-被动分离（AIS）方法，以从空间实验的栖息地笼单元中获取的视频中提取活动和不活动。该方法通过“整个活动中活动的比例”来得到活动比，通过分析适应不同重力条件的情况（1g 到超重力（HG），以及从微重力（MG）到人工 1g（AG）），来计算活动比和活动间隔。使用 AIS 对地面控制实验的结果与之前的研究接近，表明了该方法的有效性。在重力从 1g 变为 HG 的情况下，在离心开始时比例较低，在第一周内急剧恢复，并且在另一周内进入稳定期。AG 和 HG 的趋势相同；适应不同的重力环境需要时间。
