Department of Medical Biochemistry, School of Pharmacy, Iwate Medical University, Morioka, Iwate, Japan.
Department of Pharmacology, Iwate Medical School, Morioka, Iwate, Japan.
Mol Cell Biochem. 2021 May;476(5):2021-2028. doi: 10.1007/s11010-021-04068-7. Epub 2021 Jan 29.
Gicerin/CD146 is a cell adhesion molecule which belongs to the immunoglobulin (Ig) superfamily. We have reported the existence of gicerin/CD146 in the nervous system, heart, lung and smooth muscles of blood vessels. In this study, we make a cardiac hypertrophy model rat by constricting the rat aorta (AAC, ascending aortic constriction) and examined the effect on the expression of gicerin/CD146 in the heart. We found that the expression level of gicerin/CD146 was increased by the AAC treatment. Next, stretch stimulation was applied to myocardial cell line H9c2 cells to confirm that gicerin/CD146 may participate in the cellular hypertrophy model. We also treated the cells with inhibitors of MAP pathway enzymes. In cultured myocardial cells, the expression level of gicerin/CD146 was increased by the stretch stimulation and decreased by inhibiting the MAP pathway. Based on the above findings, it is suggested that the expression of gicerin/CD146 is involved in cardiac hypertrophy, and that the MAP pathway may be involved in the expression of gicerin/CD146 RNA in the cardiomyocyte. In addition, the expression level of gicerin/CD146 RNA in neonatal rats was upregulated after birth. Therefore, it is suggested that gicerin/CD146 might participate in the increase of myocardial cell volume both in the pathway of cardiac hypertrophy and in the developmental growth of heart.
Gicerin/CD146 是一种细胞黏附分子，属于免疫球蛋白（Ig）超家族。我们曾报道过 Gicerin/CD146 存在于神经系统、心脏、肺和血管平滑肌中。在这项研究中，我们通过缩窄大鼠主动脉（AAC，升主动脉缩窄）建立了心肌肥厚大鼠模型，并检测了 Gicerin/CD146 在心脏中的表达变化。结果发现，AAC 处理后 Gicerin/CD146 的表达水平增加。接下来，我们对心肌细胞系 H9c2 细胞进行拉伸刺激，以确认 Gicerin/CD146 是否参与了细胞肥大模型。我们还使用 MAP 通路酶抑制剂处理细胞。在培养的心肌细胞中，拉伸刺激可增加 Gicerin/CD146 的表达水平，而抑制 MAP 通路则可降低其表达水平。基于上述发现，我们推测 Gicerin/CD146 的表达参与了心肌肥厚，MAP 通路可能参与了心肌细胞中 Gicerin/CD146 RNA 的表达。此外，新生大鼠出生后 Gicerin/CD146 RNA 的表达水平上调。因此，我们推测 Gicerin/CD146 可能参与了心肌细胞体积的增加，既参与了心肌肥厚的通路，也参与了心脏的发育生长。
