Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, Anhui Provincial Engineering Laboratory of Water and Soil Pollution Control and Remediation, School of Ecology and Environment, Anhui Normal University, Wuhu 241003, PR China.
Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, Anhui Provincial Engineering Laboratory of Water and Soil Pollution Control and Remediation, School of Ecology and Environment, Anhui Normal University, Wuhu 241003, PR China.
Sci Total Environ. 2021 May 20;770:145201. doi: 10.1016/j.scitotenv.2021.145201. Epub 2021 Jan 22.
Recently, considerable progress has been made in the environmental application of nanotechnology. However, little is known about how nanomaterials might affect the cyanobacterial suppression potential of allelochemicals. In this study, a microcosm was employed to simulate and verify the effect of magnetic FeO nanoparticles (MFN) on the inhibitory influence of allelopathic hydroxybenzoic acid (p-Ha) on bloom-forming Microcystis aeruginosa. MFN had a hormetic effect on cyanobacterial growth. At a neutral concentration of 182 mg/L, MFN enhanced the algal suppression by p-Ha and decreased the IC by half, which was significantly and positively associated with the amount of OH. Furthermore, adding MFN induced a stronger physiological response than treatment with only p-Ha. The cellular integrity was severely disrupted for the cyanobacterium M. aeruginosa. The total protein content decreased rapidly to inactivate the algae by limiting the amounts of extracellular microcystin and polysaccharide released. The modification of the effect of p-Ha by MFN was reflected by the intracellular NO content of M. aeruginosa. In addition, the typical radical scavengers ascorbic acid and 5,5-dimethyl-1-pyrroline N-oxide decreased OH production to weaken algal suppression under the combined treatment with p-Ha and MFN. By contrast, the addition of Fe and increasing the light intensity triggered the generation of OH and strong cyanobacterial suppression. Thus, MFN could enhance the cyanobacterial control efficiency of p-Ha and decrease the input of allelochemicals in the field. These findings suggest a novel mode of allelochemical modification by nanomaterials as a promising cyanobactericide for harmful algal bloom management.
最近，纳米技术在环境应用方面取得了相当大的进展。然而，对于纳米材料如何影响化感物质对蓝藻的抑制潜力知之甚少。在这项研究中，采用微宇宙模拟并验证了磁性 FeO 纳米粒子（MFN）对化感羟基苯甲酸（p-Ha）对形成水华的铜绿微囊藻抑制影响的作用。MFN 对蓝藻生长具有毒物兴奋效应。在中性浓度 182mg/L 时，MFN 增强了 p-Ha 的抑藻作用，并将 IC 降低一半，这与 OH 的数量呈显著正相关。此外，与仅用 p-Ha 处理相比，添加 MFN 会引起更强的生理响应。铜绿微囊藻的细胞完整性受到严重破坏。总蛋白含量迅速下降，通过限制胞外微囊藻毒素和多糖的释放来使藻类失活。MFN 对 p-Ha 作用的修饰反映在铜绿微囊藻的细胞内 NO 含量上。此外，作为典型的自由基清除剂抗坏血酸和 5,5-二甲基-1-吡咯啉 N-氧化物降低了 OH 的产生，从而在 p-Ha 和 MFN 联合处理下削弱了对藻类的抑制作用。相比之下，添加 Fe 和增加光照强度会引发 OH 的产生并强烈抑制蓝藻。因此，MFN 可以增强 p-Ha 对蓝藻的控制效率，并减少田间化感物质的投入。这些发现表明，纳米材料可以通过一种新的化感物质修饰模式，作为一种有前途的蓝藻抑制剂，用于有害藻类水华的管理。
