Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Iran.
Department of Horticultural Science, Imam Khomeini International University, Qazvin, Iran.
Food Chem. 2021 Jun 30;348:129074. doi: 10.1016/j.foodchem.2021.129074. Epub 2021 Jan 19.
Exogenous melatonin application at 0, 1, 10, 100, and 1000 µM retarded cap browning of button mushrooms (Agaricus bisporus) by 78.35, 31.40, 30.91, 27.17, and 32.50 %, respectively.Mushrooms treated with 100 µM melatonin also had lower weight loss and higher firmness. During the first 5 days of storage at 4 °C, higher HO accumulation may serve as a signal for promoting endogenous melatonin accumulation by triggering the expression of TDC, T5H, SNAT, and ASMT genes, beneficial for preserving membrane integrity. Besides, the higher accumulation of phenols in mushrooms treated with 100 µM melatonin may be ascribed to higher PAL and lower PPO gene expression and enzyme activity. Moreover, higher DPPH scavenging capacity in mushrooms treated with 100 µM melatonin may be ascribed to the higher accumulation of phenols and ascorbic acid.
外源性褪黑素在 0、1、10、100 和 1000μM 时分别使双孢蘑菇（Agaricus bisporus）菌盖褐变延缓了 78.35%、31.40%、30.91%、27.17%和 32.50%。用 100μM 褪黑素处理的蘑菇的失重率也更低，硬度更高。在 4℃下储存的前 5 天，较高的 HO 积累可能作为一种信号，通过触发 TDC、T5H、SNAT 和 ASMT 基因的表达，促进内源性褪黑素的积累，有利于保持膜的完整性。此外，用 100μM 褪黑素处理的蘑菇中酚类物质的积累较高，可能归因于 PAL 基因表达和酶活性降低，而 PPO 基因表达和酶活性升高。此外，用 100μM 褪黑素处理的蘑菇的 DPPH 清除能力较高，可能归因于酚类物质和抗坏血酸的积累。
