Department of Horticultural Science, Imam Khomeini International University, Qazvin, Iran.
Zhejiang University, College of Biosystems Engineering and Food Science, Key Laboratory of Agro-Products Postharvest Handling Ministry of Agriculture, Hangzhou 310058, People's Republic of China.
Food Chem. 2019 Sep 30;293:285-290. doi: 10.1016/j.foodchem.2019.05.002. Epub 2019 May 2.
Exogenous adenosine triphosphate (ATP) treatment at 0, 250, 500, 750, and 1000 µM retarded cap browning in mushrooms by 0, 34, 26, 51 and 32 %, respectively, during storage at 4 °C for 18 days. Triggering signaling HO accumulation arising from elevating NADPH oxidase enzyme activity during 6 days of storage at 4 °C may be pivotal for promoting shikimate dehydrogenase enzyme activity in mushrooms treated with ATP during 18 days of storage at 4 °C. Promoting melatonin accumulation (390 µg kg FW vs. 160 µg kg FW) in mushrooms treated with ATP during cold storage may attribute to signaling HO accumulation. Higher DPPH scavenging capacity (72 % vs. 65 %) in mushrooms treated with ATP may attribute to higher phenols accumulation arising from higher phenylalanine ammonialyase/polyphenol oxidase enzymes activity concomitant with higher alternative oxidase gene expression during 18 days of storage at 4 °C.
在 4°C 下储存 18 天时，0、250、500、750 和 1000µM 的外源三磷酸腺苷 (ATP) 处理分别使蘑菇的帽褐变延迟了 0、34、26、51 和 32%。在 4°C 下储存 6 天时，NADPH 氧化酶活性升高引发 HO 积累的信号触发，可能对在 4°C 下储存 18 天时用 ATP 处理的蘑菇中的莽草酸脱氢酶活性具有促进作用。在冷藏过程中用 ATP 处理的蘑菇中，褪黑素积累（390µg•kg FW 比 160µg•kg FW）增加，可能归因于 HO 积累的信号触发。在 4°C 下储存 18 天时，用 ATP 处理的蘑菇的 DPPH 清除能力（72%比 65%）更高，这可能归因于苯丙氨酸解氨酶/多酚氧化酶活性升高，同时交替氧化酶基因表达更高，导致酚类物质积累更高。
